Study of Some Biomarkers and HLA-G for Early Detection of Multiple Sclerosis Disease

Abstract

Multiple sclerosis is the most commonest a chronic inflammatory, demyelinating and neurodegenerative
disorder in the central nervous system (CNS) of the brain and spinal cord. The disease commonly start onset
in the age of (20-50)years, as for women, they are at greater risk of MS than men. The aim of this dissertation
is to detected some biomarkers(IL-18, Caspase-1 and Opioid growth factor) in the serum and saliva in
patients with MS and study the HLA-G genotype. Moreover, the current study indicated a comparison
between the levels of biomarkers in two groups for MS patients (early diagnosis of MS without treatment
and with treatment group) with the group of healthy controls. This study included the total number 150
case, it was classified to the group I early diagnosis without treatment (54 patients) , the group II using
treatment (46 patients)and the group III (50) healthy controls. This study included its own questionnaire
structural, where it includes the following: gender and age. Biomarkers were examined by ELISA methods
and (HLA-G) it was done by PCR,PCR Sequencing methods. The age mean +SD of the untreated group
of new early diagnosis of MS was recorded (35.54 ± 8.51) and the treated group MS (34.22 ± 8.24) while
(36.5 ± 8.93) in normal population controls. This study showed the concentrations(IL-18, Caspase-1 and
OGF) in the serum and saliva of MS patients for both groups comparison healthy controls. This study was
conducted using a ROC chart for patients with MS in Iraq for biomarkers for both groups. In the current
study investigated the effect of HLA-G 14bp polymorphism on MS. As well as, to genotype (+ 3142G>