Time-dependent Expression of Caspase-3 and Degeneration of Lateral Rectus Muscle on Experimental Esotropia in Rabbits

Authors

  • Luki Indriaswati
  • Nurwasis
  • Gatut Suhendro
  • Soetjipto
  • Retno Handajani

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14785

Keywords:

caspase-3, degeneration of lateral rectus muscle, esotropia, rabbit

Abstract

Background: To identify correlation between caspase-3 expression and degeneration of lateral rectus muscle
on experimental esotropia in rabbits by using time dependent analysis. Methods: Twenty four rabbits were
divided into 4 groups, first month sham (S1); second month sham (S2); first month esotropia (E1); and
second month esotropia (E2) model groups. Immunohistochemical and haematoxyllin eosin staining were
performed. Results: In first month observation no significant difference in caspase-3 expression between S1
(1.56 ± 1.03) and E1 (3.93 ± 1.66) groups (p >0.05) and in the degeneration score of lateral rectus muscle
between S1 (1.33 ± 1.36) and E1 (3.83 ± 2.04) groups (p> 0.05). In second month observation no significant
difference in caspase-3 expression between S2 (1.80 ±1.08) and E2 (6.50 ± 3.26) groups (p >0.05); but
there was significant difference in the degeneration score of lateral rectus muscle between S2 (1.16 ± 1.16)
and E2 (4.50 ± 1.04) groups (p <0.05). There was a significant correlation between caspase-3 expression
and the degeneration score of lateral rectus muscle (p=0.020; r=0.471). Conclusion: On the experimental
esotropia with prolonged lateral muscle contraction in rabbits, the increasing trend of caspase-3 expression
has a correlation with the increasing degeneration score of lateral rectus muscle.

Author Biographies

Luki Indriaswati

Doctoral student

Nurwasis

Lecturer

Gatut Suhendro

Professor, Department of Ophthalmology

Soetjipto

Professor

Retno Handajani

Professor, Department of
Biochemistry, Faculty of Medicine, Universitas Airlangga

Published

2021-03-24

How to Cite

Luki Indriaswati, Nurwasis, Gatut Suhendro, Soetjipto, & Retno Handajani. (2021). Time-dependent Expression of Caspase-3 and Degeneration of Lateral Rectus Muscle on Experimental Esotropia in Rabbits. Indian Journal of Forensic Medicine & Toxicology, 15(2), 2730-2735. https://doi.org/10.37506/ijfmt.v15i2.14785