Genotyping of Human Papilloma Virus Causing Skin Wart among Iraqi Patients

Authors

  • Mohammed Ghani Madlu
  • Wissam Saleh Abood

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14807

Keywords:

HPV, Wart skin disease , L1 gene, RT-PCR .

Abstract

The papillomaviruses are a large group of tiny, non-enveloped DNA viruses that in several different
anatomical locations can cause squamous epithelial tumor’s (warts and papilloma’s). There is a close
association between HPV and cervical uterine cancer . The genome is composed of 7200–8000 base pairs
and is divided into three parts, early region (E) comprising of E1, E2, E4–E8 and reflecting 50 percent of the
L1 and L2 genome, Genomic DNA from wart tissue samples were extracted by using gSYAN DNA mini kit
extraction kit . The Real Time PCR primers for detection and genotyping of DNA Human papillomavirus
type 1 and type3 and type6 based on L1 gene that designed in this study using NCBI-Genbank Sequence
database and primer 3 plus . The genetic study included analysis of a common DNA to HPV that was found
in 20 % of cases and DNA fragment specific to HPV type 1, 3 and 6 based on real time PCR. Type 1 HPV
was not approved to present in any sample in the study. Type 3 HPV was identified in a single case. HPV 6
was identified in 8 cases, Therefore, type 6 HPV is the most common genotype, followed by type 3.

Author Biographies

Mohammed Ghani Madlu

Research Scholar, Corresponding Author, University of Al-Qadisiyah / College of
Medicine, Iraq

Wissam Saleh Abood

Asst. Professor, University of Al-Qadisiyah, College of Medicine, Iraq

Published

2021-03-24

How to Cite

Mohammed Ghani Madlu, & Wissam Saleh Abood. (2021). Genotyping of Human Papilloma Virus Causing Skin Wart among Iraqi Patients. Indian Journal of Forensic Medicine & Toxicology, 15(2), 2881-2886. https://doi.org/10.37506/ijfmt.v15i2.14807