Detection of Mycotoxigenic Fungi on Food from Markets with the Polymerase Chain Reaction Method for Halal and Thoyiban Food
Keywords:Aflatoxin B, mycotoxins, traditional market, supermarket.
Secondary metabolites of filamentous mold are mycotoxins, which in some situations can develop on foods
derived from plants or from animals. Fusarium, Aspergillus, and Penicillium are the most common types of
mold that produce mycotoxins and also often contaminate human food and animal feed. Aflatoxins including
aflatoxin B1, B2, G1 and G2 are produced by A. flavus and A. parasiticus. M1 and M2 aflatoxins are
found in dairy products. In this study, we used PCR to detect and identify mycotoxigenic fungi material in
foods from traditional markets and supermarkets in Surabaya, Indonesia. Samples of chicken meat from a
traditional market and from a supermarket (10 pieces each) were placed in a conical tube and crushed in PBS.
The crushed samples were centrifuged and the supernatants were cultured on potato dextrose agar (PDA)
media and observed using a reverse microscope. DNA was isolated from cultured samples and subjected
to PCR with primers specific for genes encoding aflatoxins. Interestingly, we revealed that the Polymerase
Chain Reaction (PCR) analysis showed A. flavus and A. ochraceus were present on chicken meat sold at
traditional markets and supermarkets. In sum, enhanced precautions may be needed to ensure that foods sold
in traditional markets and supermarkets are free from molds that have the potential to produce mycotoxins.
Further studies are needed to detect and identify the prevalence of mycotoxins in the food supply.
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Re use and mixing of content policy- We follow Creative Commons Licence Policy. We follow CC BY. Please refer below for all details
This license lets others distribute, remix, adapt, and build upon our work, even commercially, as long as they credit us for the original creation.
- The journal allows readers to read, download, copy, distribute, print, search, or link to the full texts of its articles and allow readers to use them for any other lawful purpose.
- The journal allows the author(s) to hold the copyright without restrictions.
- The journal allows the author(s) to retain publishing rights without restrictions