Phylogrouping, Serogrouping, Virulence Factors and Carbapenemase Genes among Carbapenemase-Producing Escherichia Coli From Urinary Tract Infections

Authors

  • Ahmed A. Mhawesh
  • Daniah Muneam Hamid
  • Abdolmajid Ghasemian

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14832

Keywords:

Escherichia coli, carbapenemase, phylogroups, serogrouping, virulence typing

Abstract

Introduction: Escherichia coli is among major nosocomial pathogens causing urinary tract infections
(UTIs). The emergence of carbapenem- resistant strains is a major concern regarding the UTI treatment. The
subjective of this study included assessment of genetic relation and screening of virulence factors among
carbapenemase producing E. coli from UTI.
Materials and methods: Three-Hundred E. coli isolates were enrolled. Antibiotic susceptibility test was
conducted by disk diffusion as provided by clinical and laboratory standards institute (CLSI). Carbapenemase
production and presence of bla OXA-48, bla KPC, bla NDM, bla VIM and bla IMP genes were evaluated by PCR
technique. Virulence factors genes were also screened by PCR technique. Genetic relation of isolates was
implemented using phylogrouping and serogrouping.
Results: Of 300 isolates, 11 (3.66%) of them were resistant to carbapenems (CR-E. coli). Imipenem
minimum inhibitory concentration ranged 4-128µg/ml. The bla OXA-48 and blaIMP genes were co-existed in
three (1%) isolates (imipenem MIC: 64-128µg/ml), but the bla KPC, bla NDM and bla VIM were not amplified.
Predominant virulence genes included iutA (n=293, 97.66%), fyuA (n=256, 85.33%), inh (n=249, 83%), traT
(n=247, 82.33%), pap? (n=96, 32%), fimH (n=93, 31%), csgA (n=92, 30.66%). All the CR-E. coli contained
the iutA, fyuA, traT, papII, fimH and csgA genes. O1 (32%), O16 (15%) and O25 (7%) serogroups were
predominant and 6/11, 4/11 and 1/11 of CR-E. coli belonged to O1, O25 and O75 serogroups, respectively.
Among eleven CR-E. coli isolates, nine of them were belonged to the B2 phylogroup and two isolates were
belonged to B1 phylogroup.
Conclusion: CR-E. coli contained the blaIMP and blaOXA-48 genes and predominantly O1 serogroup. High
rate of virulence factors among CR-E. coli from UTI is a concern in Baghdad hospitals. The spread of
isolates with resistance to last-line antibiotics must be controlled.

Author Biographies

Ahmed A. Mhawesh

Asst. Prof., Dept. of Med. and Mol. Biotech. -Coll. of Biotech. - Al-Nahrain University/Baghdad /Iraq

Daniah Muneam Hamid

Asst. Lecturer DNA Forensic Center for Research and Training/AL-Nahrain University/Baghdad/ Iraq

Abdolmajid Ghasemian

Lecturer,
Islamic Azad University, Central Tehran Branch/Tehran/Iran

Published

2021-03-24

How to Cite

Ahmed A. Mhawesh, Daniah Muneam Hamid, & Abdolmajid Ghasemian. (2021). Phylogrouping, Serogrouping, Virulence Factors and Carbapenemase Genes among Carbapenemase-Producing Escherichia Coli From Urinary Tract Infections. Indian Journal of Forensic Medicine & Toxicology, 15(2), 2997-3003. https://doi.org/10.37506/ijfmt.v15i2.14832