Study the Effect of Ultraviolet (UV) on Mutagenicity of Pseudomonas Aeruginosa Isolated from Burn Wound Infections
Keywords:ultraviolet, mutagenicity, pseudomonas aeruginosa
Burn wound infections are regarded as one of the serious complications occurs in the acute period after
injury and leads to high morbidity and mortality. Pseudomonas aeruginosa plays a prominent role as an
etiological agent involved in serious infections of burn and wounds.
During this study, a total of (52) samples were taken from patients suffering from burn wound infections
in (2) hospital in Baghdad from 1/9/2019 to 1/12/2019 for isolation of pseudomonas aeruginosa. Results
reveals that (31) isolate (59.6%) were gave typical morphological characteristics and biochemical test that
related to Pseudomonas aeruginosa. Five Pseudomonas aeruginosa isolates were subject to antimicrobial
susceptibility test that was done according to NCCLS. Results of Antibiotic susceptibility test for pseudomonas
aeruginosa isolates reveals that 100% of them were sensitive to ciprofloxacin and Norfloxacin while 100%
of the isolates were resistant to ampicillin ,cefixime ,Erythromycin, Naldixic acid , chloramphenicol and
Mutagenesis of Pseudomonas aeruginosa PS1 isolate by UV irradiation was done for (60,120,240,300 ) Sec.
Results of mutation to ( PS1) isolate revealed that exposure to UV irradiation from (60-300) Sec leads to decrease
in the viability of bacterial cells but increase in the sensitivity of PS1 isolate to ciprofloxacin,gentamycine,
Norfloxacin and oxytetracycline that reach to (40,21,26,30 )mm zone of inhibition respectively . The
same isolate revealed changes in results from resistant to sensitive to chloromphenicol ,Erythromycin and
Naldixic acid that reach to (33,31,33)mm zone of inhibition respectively but its gave the same results for the
antibiotics (Ampicillin,Bacitracin and cefixime).
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