Detection Trisomy Chromosome 12 in Relation to the Expression Of Cd38 and Light Chain Restriction in Chronic Lymphocytic Leukemia Patients
Keywords:leukemia patients ; light chain restriction; trisomy chromosome 12 ; toxicity; health behavior
Background: Chronic lymphocytic leukemia is a malignancy of mature B cells where there is progressive
accumulation of leukemic cells. The genetic factors have been found to play a role, deletions of the long arm
of chromosome 13, specifically involving band 13q14 (del(13q14)), represent the single most frequently
observed cytogenetic aberration in CLL, followed by trisomy 12 ,del 11q22, del 6q21, del 17p13, and 14q.
Objectives: To detect the structural abnormalities on chromosome 12 (trisomy 12 in patients with CLL
by using FISH technique; To investigate the relation of this chromosomal abnormalities with with the
expression of CD38 and light chain restriction .
Patients, Materials and Methods: 1. This cross-sectional study was conducted from February 25, 2018 to
December 15, 2019. Fifty newly diagnosed adult patients with CLL were enrolled in this study. 2. Each patient
was assessed at the time of admission, full clinical and laboratory data were obtained from patient’s hospital
records. Three ml of fresh blood sample were taken from each patient at admission. Upon confirmation of
CLL diagnosis, samples were taken to FISH unit for blood preparation which includes two steps in two
days, the first day to separate lymphocyte cells from another component of blood and second-day washing
samples and stored till slide preparation. Slide preparation done in the 1st day and using dual color gene
probe and slide reading in the second day by fluorescent microscope. The collected data were computerized
and statistically analyzed using the SPSS program (Statistical Package for Social Science) version 23.
Result: The median age of the patients was 64 years and the range was 45-76 years. Among 50 patients
studied, 18 patients were females, representing (36%) and 32 patients were males representing (64%). Male:
female ratio 1.8:1. Trisomy chromosome12 was found 15 out of 50 patients with CLL, representing 30%.
Conclusion:1.In the current study,15 out of 50 CLL patients (30%)present with trisomy chromosome 12 in
a sample of Iraqi adult CLL patients.2. trisomy CH 12 patients show significant relation with light chain
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