The Role of Vitamin D3 as an Antioxidant and Hepatic Treatment against the Toxic Effect of Cadmium Chloride in The Rats

Authors

  • Baraa yaqoob Al-Rahoo
  • Banan Rakan Dabdoub

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14839

Keywords:

Antioxidant; Hepatotoxicity; Vitamin D3; Cadmium chloride; Free radical

Abstract

Current study experiments were conducted on male Rattus rattus norvigicus, at age of two months, with the
weight of (200-250gm) divided into 8 groups for each group of 8 rats. The lethal average dose of (LD50) for
cadmium chloride and the choice of bodyweight (5mg/kg) to inject rats Intraperitonially were determined,
then orally doses of vitamin D3 after a week of CdCl2 injection at a concentration of 1000IU/kg bodyweight
at three doses per week as well as for the rest of the concentrations, 2000IU/kg bodyweight, and 4000IU/kg
bodyweight. After four weeks of injection and administration, blood was drawn from the rats in each group,
then the rats were slaughtered and the liver was taken for tests. The current study aimed to use different
concentrations of vitamin D3 as a treatment, the antioxidant in the liver of rats injected with heavy metal
CdCl2. Due to the study, some biochemical, physiological, and tissue changes in them through the study of
liver function for rats experimenting with transactions, in addition to studying the level of oxidative stress
and antioxidants in the liver. The results of the current study have shown that the role of vitamin D3 as an
antioxidant and as a treatment in reducing the toxic effect of CdCl2 to a very large. The best concentration of
vitamin D3 used in the case study was 2000 IU/kg bodyweight, which was taken for rats and three doses per
week for four weeks, and after being injected with CdCl2, there was a significant decrease at the probability
level P?0.05 for the concentration of the ALT when compared to the control group. On the other hand, there
is a significant rise in glutathione (GSH) and catalase (CAT) levels in rat liver, at the same concentration
of vitamin D3, when compared with the control group, while there was a clear improvement in AST and
malondialdehyde (MDA) in the liver, the results were very similar to those of the control group. Perhaps
vitamin D3 dosage of cadmium-injected rats may protect improving the toxic effect of CdCl2 in the liver.

Author Biographies

Baraa yaqoob Al-Rahoo

Post graduate

Banan Rakan Dabdoub

Lecturer/ College of Education for Pure Sciences, University of Mosul, Iraq

Published

2021-03-24

How to Cite

Baraa yaqoob Al-Rahoo, & Banan Rakan Dabdoub. (2021). The Role of Vitamin D3 as an Antioxidant and Hepatic Treatment against the Toxic Effect of Cadmium Chloride in The Rats. Indian Journal of Forensic Medicine & Toxicology, 15(2), 3047-3052. https://doi.org/10.37506/ijfmt.v15i2.14839