Detection of Capsular Polysaccharide Virulence Genes rmpA and magA of Klebsiella Pneumonia Isolate from Diabetic Foot Ulcer Patient in Najaf Governorate in Iraq
Keywords:patients; Capsular polysaccharide; genes rmpA; and magA of Klebsiella pneumonia; toxicity
Klebsiella Pneumoniae is an opportunistic pathogen, which mostly cause nosocomial infections. K.
Pneumoniae utilizes a variety of virulence factors, especially capsule polysaccharide, lipopolysaccharide,
fimbriae, outer membrane proteins and determinants for iron acquisition and nitrogen source utilization,
for survival and immune evasion during infection. A total of (36) clinical swab specimens isolated from
patient with diabetic foot ulcer in Al-Sdder Medical City (Central Diabetic Foot) in Najaf Governorate,
Iraq from November 2019 to February 2020 , for detection some virulence capsular polysaccharide genes
such as rmpA and magA that related with K1 serotype of K . pneumonia and associated with antibiotic
resistance pattern in patient of diabetic foot ulcer. Antibiotic susceptibility test of K. Pneumoniae isolates
against (20) of commonly used antibiotic showed there 25 (69%) of K. pneumonia isolate from diabetic
foot patient were Multi-drug resistance (MDR),while11(31%) were extensive-drug resistance (XDR). The
results of amplified product demonstrated that K1 serotype of K . Pneumonia was positive in 23 (64%)
of totally clinical specimen than other types of serotype ( Non-K1 serotype). (magA) gene that associated
with hypermucoid viscosity was positive in 21 (58%) of total specimens while (rmpA) gene that regulate of
capsular polysaccharide of K .pneumonia was positive in 22 (62%) of totally samples
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