BCL-2 Gene Expression association with Breast Cancer Susceptibility of Iraq Population
Keywords:Breast Cancer, real-time PCR , Bcl-2 gene.
Breast cancer is a significant illness in the world. Molecular methods are important and essential for the
diagnosis of breast cancer. Many of the genes with expression modification, such as the Bcl-2 gene, are
explicitly encoding an anti-apoptotic protein and thus known as oncogene.A total of 55 patients with breast
cancer divided into( 25 Stage 0 , 15 Stage IA, and15 Stage IB), and 25 control group were enrolled in this
study, age range of 35 – 60 years old the samples were collected from Al-Eluia hospital for woman care,
oncology teaching hospital.RNA were extracted and then converted to cDNA to perform real time- PCR
done for those samples to detect the gene expression.Expression of gene in the study is higher levels in
malignant group stage IB, following stage IA ,and stage0 and the fold of expression was 10.00 time higher
than the control group, significantly higher in the third grade of breast tumor samples with Ct (22.14) of the
first grade with CT (25.63) and the second with CT (24.07). According to the results of the study that the use
of molecular methods in measuring the expression of Bcl-2 gene may help to diagnose the disease and may
be considered that the Bcl2 gene is molecular tool for the early detection of breast cancer.
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