Contaminant Investigations of Regularly used Eye Contact Lenses


  • Rebwar Muhammad Hamasalih
  • Karzan Abdulmuhsin Mohammed
  • Pinar Khalid Khudhur



Biofilms; contact lenses; antibiotic discs.


Microorganisms, particularly bacteria, are considered to play a role in the etiology of certain corneal infections
observed during soft contact lens wear. This study explored the possibility of microbial colonization of soft
contact lenses as a risk factor leading to corneal infection. In a clinical trial conducted and 86 lenses were
recovered aseptically and analyzed for microbial colonization. The incidence of bacteria and fungi was
fewer during corneal infection than during asymptomatic lens wear. During asymptomatic lens wear, corneal
infection bacteria were isolated most frequently and were usually normal external ocular microbiota. Of the
Gram-positive bacteria, the incidence of Staphylococcus aureus was fewer during asymptomatic wear. While
Gram-negative bacteria were seen in high cases during asymptomatic wear, this includes Escherichia coli
and Pseudomonas aeruginosa. Fungal screening and identification revealed 2 cases of contamination with
the yeast Candida albicans that able to form a biofilm. Colonization of soft contact lenses with pathogenic
bacteria, especially gram-negative and gram-positive bacteria, appears to be a significant risk factor leading
to corneal infection.

Author Biographies

Rebwar Muhammad Hamasalih

PhD of Molecular Biology, Department of Biology, Salahaddin University-Erbil, Erbil, Iraq

Karzan Abdulmuhsin Mohammed

Assistant Professor,
Scientific Research Centre, Salahaddin University-Erbil, Erbil, Iraq

Pinar Khalid Khudhur

MSc of Medical Microbiology, Hawler
Medical University/ College of Medicine/Basic Science Department/Microbiology Unit



How to Cite

Rebwar Muhammad Hamasalih, Karzan Abdulmuhsin Mohammed, & Pinar Khalid Khudhur. (2021). Contaminant Investigations of Regularly used Eye Contact Lenses. Indian Journal of Forensic Medicine & Toxicology, 15(2), 3264-3275.