Antimicrobial Activity of Lactic Acid Bacteria Found in Dadiah on Disease-Causing Skin Infections

Abstract

Dadiah is a fermented food made from buffalo milk containing lactic acid bacteria (LAB). The potential
of LAB as an antimicrobial organism is expected to inhibit or kill pathogens, especially those causing
inflammations in the skin. Dadiah used in this study was obtained from the Lintau area of Tanah Datar
District, West Sumatra, Indonesia. LAB isolation and identification involved the use of MRS Broth
(MERCR) and MRS Agar (MERCK) media and the pathogenic bacteria used include Staphylococcus
aureus, Propionibacterium acnes, Acinetobacter Baumannii, Klebsiella pneumonia, and Pseudomonas
aeruginosa. The results of this study obtained a total LAB of 7x1011 CFU / g and showed the highest
antimicrobial activity in the Staphylococcus aureus with a14.53 mm clear zone and 13.18 mm bacteria in
the Pseudomonas aeruginosa test bacteria.