Hematological Alterations Induced by Zinc Oxide Nanoparticles and Preventive Role of Moringa Oleifera: In vivo

Abstract

Increasing applications on nanomaterials have raised concerns about the toxicity of these materials
toward human health. This study aims to illustrate the effects of Zinc Oxide Nanoparticles (ZnONPs) on
hematological parameters in rats and to explore the protective action of Moringa oleifera (MO) against
hematological changes induced by ZnONPs in rats. In our study, (24) albino rats were divided into four
groups (6 for each group) and used. The studied rats included rats which received 100 mg/Kg ZnONPs for
60 days , while the control group were rats without treatment. In the ZnONPs+MO group, rats were coadministered with a combination of ZnONPs at a 100mg/kg dose with MO at a 300 mg/kg dose for 60 days.
The MO group animals were treated with 300 mg/kg of MO for 60 days. The animals were sacrificed and
blood samples were obtained by cardiac puncture in the end of the experiments. Various blood parameters
were measured using HORIBA-Medical automatic hematology analyzer. Rats treated with ZnONPs showed
a significant decrease (p<0.05) in red blood cell counts (RBCs), hemoglobin (Hb) concentration, mean
corpuscular volume (MCV), mean corpuscular haemoglobin (MCH), mean corpuscular haemoglobin
concentration (MCHC), hematocrit value (Hct), and platelet count (Plt) count, except white blood cell count
(WBCs) in comparison to the control group. Then, co-administration of M. Oleifera along with ZnONPs
exerted a significant recovery effect in hematological disorders. Thus, these consequences established that
M. Oleifera shown a defensive impact against ZnONPs induced hematological alterations in male rats.