Evaluation of Biofilm Formation in Klebsiella Pneumoniae and Antibiotic Resistance

Authors

  • Sara Hadi Jassim
  • Anfal Shakir Motib

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14901

Keywords:

Klebsiella pneumoniae, UTI, antibiotic resistance, biofilm formation.

Abstract

Biofilm are densely packed communities of germ cell (bacterial cells), that grow either on living or inert
surface, surround themselves with secreted polymers, the role of biofilm formation and development by
bacteria has been suggested to be an important stage in the pathogenesis of numerous bacterial species.
In this study klebsiella pneumoniae has been investigated to form biofilms and its relation to antibiotics
resistant. The antibiotics which are used in this study are Ciprofloxacin (CIP) and Cefotaxime (CTX) because
most of the isolates showed resistance to these antibiotics. The experiment was design to check the cause
of UTI in the different types of patient by taking their urine as a specimen sample. Out of 160; 24 samples
showed Klebsiella pneumoniae, which cause the urinary tract infection. The organisms were identified
by the DNA isolation and checking with PCR using 16SrRNA. Antibiotic susceptibility for the different
groups of antibiotic were done by using disk diffusion method against all Klebsiella pneumoniae isolates.
Klebsiella pneumoniae are important infectious bacterium and become more dangerous with their capacity
of multi-drug resistant. Therefore, there is need to find out alternate therapy to eradicate the infection of this
bacterium by reduce the formation of biofilm which may lead to decrease the antibiotics resistance.

Author Biographies

Sara Hadi Jassim

Biologist, Department of Microbiology, College of Medicine/ University of Diyala, Iraq

Anfal Shakir Motib

Assistant Professor
Doctor, Department of Microbiology, College of Medicine/ University of Diyala, Iraq

Published

2021-03-24

How to Cite

Sara Hadi Jassim, & Anfal Shakir Motib. (2021). Evaluation of Biofilm Formation in Klebsiella Pneumoniae and Antibiotic Resistance. Indian Journal of Forensic Medicine & Toxicology, 15(2), 3413-3419. https://doi.org/10.37506/ijfmt.v15i2.14901

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