Melatonin Receptor A Gene, Melatonin Receptor C Gene and Ovocalyxin-32 gene polymorphisms associated with some Egg Production in Local Iraqi Chicken

Authors

  • Israa Luay Hamdan AL-Jaryan
  • Walaa Salih Hassan
  • Muhannad Munthir AL-Rekabi

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14939

Keywords:

Local Iraqi chicken, genetic polymorphism, Melatonin receptor c, egg production traits, Local chicken, crossbreeding, breeding poultry and improvement, Melatonin receptor a gene, ovocalyxin -32 gene, OCX-32, MTNRa

Abstract

The aim of this research is to detect genetic polymorphisms of some candidate genes associated with egg
production trait and to find the relationship between some of these genetic sites and the productive traits
of Local Iraqi chickens that will be used for the national poultry breeding programs to increase quality egg
production in endogenous chicken. Genomic DNA were extracted and then amplified three genes (melatonin
receptor a gene MTNRa, melatonin receptor c gene MTNRc and ovocalyxin-32 gene (OCX-32). (MTNRa)
gene amplified by PCR-SSCP method. The distribution of percentages in female Iraqi Local chicken studied
for (MTNRa) were 73, 20, 5 and 2% for TN, TNA, NN and TT SSCP-genotype respectively. It turned
out that phenotypic polymorphisms of MTNRa gene was significant (p<0.05) with Serum Melatonin and
Magnesium concentrations, in addition to First egg weight FEW and Body weight at maturity. (MTNRc)
gene amplified by PCR-RFLP method, the distribution of percentages of MTNRc genetic location in female
Iraqi Local chicken Flock studied were 23, 61 and 16 % for MM, Mm, mm RFLP-genotype respectively, it
turned out that phenotypic diversity of MTNRc gene was significant (p<0.05) in mean of Serum Melatonin
and Magnesium concentration, in addition to First egg weight FEW and Body weight at maturity. (OCX-32)
gene amplified by PCR-SSCP method. The distribution of percentages in female Iraqi Local chicken studied
were 98 and 2% for WS and SS (SSCP-genotype) respectively. It turned out that phenotypic polymorphisms
of OCX-32 genetic site were significant (p<0.05) with serum Melatonin and Magnesium concentration and
Body weight at maturity. The present research suggests that the genes MTNRa, MTNRc and OCX-32 could
be used as a possible marker in Marker Assisted Selection (MAS) and genomic selection programs for local
Iraqi chickens.

Author Biographies

Israa Luay Hamdan AL-Jaryan

Assistant Lecturer, College of Agriculture, Department of Animal resources, AL-Qasim Green University,
Babylon, Iraq

Walaa Salih Hassan

Assistant Professor, Department of Biology, College of Science, University of Babylon, Babylon,
Iraq

Muhannad Munthir AL-Rekabi

Assistant Professor, Animal resources and fisher’s center, Ministry of science and Technology, Bagdad, Iraq

Published

2021-03-24

How to Cite

Israa Luay Hamdan AL-Jaryan, Walaa Salih Hassan, & Muhannad Munthir AL-Rekabi. (2021). Melatonin Receptor A Gene, Melatonin Receptor C Gene and Ovocalyxin-32 gene polymorphisms associated with some Egg Production in Local Iraqi Chicken. Indian Journal of Forensic Medicine & Toxicology, 15(2), 3631-3639. https://doi.org/10.37506/ijfmt.v15i2.14939