Calorie Restriction and Moderate-Intensity Continuous Exercise Decrease Free Fatty Acid Levels and Visceral Fat Weight on High Calorie Diet Female Mice

Authors

  • Ahmad Rukhani Lutfi
  • Lilik Herawati
  • Widjiati
  • Gadis Meinar Sari

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14944

Keywords:

Calorie restriction, moderate-intensity continuous exercise, free fatty acids, visceral fat, cholesterol

Abstract

Objective: This study aimed to analyze the effect of a combination of calorie restriction and moderateintensity continuous exercise on FFA levels and visceral fat weight in female mice exposed to a high-calorie
diet.
Material and Method: This research was randomized posttest used only control group design with female
mice as the sampl Balb/c mice (Mus musculus), 8 weeks old, 20-30 grams. It was randomly divided into four
groups, namely C (control group), G1 (moderate-intensity continuous exercise group), G2 (calorie restriction
group), and G3 (combination group calorie restriction and moderate-intensity continuous exercise) where
in every group consisted six female mice. Providing a high-calorie diet using ad libitum plus D40 as much
as 3-5% of calories (0.0325 mL/gBB) for 4 weeks. The provision of a high-calorie diet was done using
the sonde technique. Calorie restriction was done 3x/week in 4 weeks by limiting calorie intake to 50% of
standard foods. The moderate-intensity continuous exercise was carried out by swimming for 15 minutes
plus 6% gBB loading, it was done 3 times/week for 4 weeks. The measurement of cholesterol, visceral fat
weight, and FFA level was done at the end of treatments.
Results: The results found that the mean of cholesterol of C C (171.00±47.74 mg/dL), G1 (126.00±15.92 mg/
dL), G2 (131.50±22.37 mg/dL), G3 (121.17±21.23 mg/dL) dan (p=0.032). The mean weight of visceral fat
at C (0.68±0.23 grams), G1 (0.25±0.08 grams), G2 (0.28±0.06 grams), G3 (0.20±0.03 grams) and (p=0.000).
The mean FFA levels were at C (1511.34±6.19 µmol/L), G1 (1419.31±53.61 µmol/L), G2 (1458.20±28.89
µmol/L), G3 (1451.55±43.77 µmol/L) and (p=0.004).
Conclusion: Based on the results of the study, it could be concluded that the provision of calorie restriction
and moderate-intensity continuous exercise significantly reduced cholesterol levels, visceral fat weight, FFA
levels and in female mice exposed to a high-calorie diet

Author Biographies

Ahmad Rukhani Lutfi

Master, Sports Health Science, Faculty of Medicine Airlangga University

Lilik Herawati

Lecturer, Department of Physiology,
Faculty of Medicine Airlangga University

Widjiati

Professor, Faculty of Veterinary Medicine, Airlangga University

Gadis Meinar Sari

Lecturer, Department of Physiology,
Faculty of Medicine Airlangga University

Published

2021-03-24

How to Cite

Ahmad Rukhani Lutfi, Lilik Herawati, Widjiati, & Gadis Meinar Sari. (2021). Calorie Restriction and Moderate-Intensity Continuous Exercise Decrease Free Fatty Acid Levels and Visceral Fat Weight on High Calorie Diet Female Mice. Indian Journal of Forensic Medicine & Toxicology, 15(2), 3665-3677. https://doi.org/10.37506/ijfmt.v15i2.14944