In Vitro Evaluation of Antimicrobial Efficacy of the Different Concentrations of Chitosan against Staphylococcus epidermidis in Disinfection of the Root Canal System
Keywords:Chitosan, Antimicrobial efficacy, S. epidermidis, root canal system
During endodontic procedures, along with mechanical instrumentation, irrigants are imperative for pathogen
eradication. As the strains of pathogens are becoming antibiotic resistant at a constant ascending pace and
also the potentially harmful effect is associated with synthetic drugs, so studies on natural alternatives for
irrigation are being done. Chitosan, a natural alternative, has been evaluated as an antimicrobial medicine.
Recently, in cases presenting with persistent infections after endodontic treatment, it was noted that of all the
microbial species identified, the prevalence of s.epidermidis was the most. In this current study, an analysis
of the antimicrobial effectiveness of chitosan as a potent root canal irrigant, for disinfecting the root canal
was conducted. Methodology: By the serial dilution method, the minimum inhibitory concentration was
determined. Dilution of the test solution was done using 10% DMSO solution. After which, incubation
in a rotary incubator, at 180 rotations per minute, the temperature of 37?, for 24 hours was done. For
obtaining the zones of inhibition of different concentration (0.2%, 0.5%, 0.75, 1%) of chitosan irrigant, the
agar diffusion method was applied and the resultant clear zone was measured with a ruler. Result: Chitosan
(liquid) had promising antibacterial activity against Staphylococcus epidermidis, with a 16 mm zone of
inhibition at 100 µg/ml. Conclusion: Within the limitations of the present study, it was observed that, even
at lower concentrations, chitosan (liquid form) effectively hindered the further growth of S.epidermidis.
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