Suitability Tests for Bacterial Identification and Antibiotic Sensitivity Tests using Microscan Walkaway on Vitek 2

Authors

  • Ria Pingkan Ganda
  • Aryati
  • Sidarti Soehita

DOI:

https://doi.org/10.37506/ijfmt.v15i2.14995

Keywords:

Identification of bacteria, antibiotic sensitivity, Microscan WalkAway, Vitek 2

Abstract

Culture or bacterial culture is the gold standard for diagnostic examination to detect the presence of
microorganisms in the patient’s body. The development of culture technology and culture-based automatic
devices for diagnostic examination are widely researched. At present, there are several types of automatic
blood culture instruments in Indonesia, namely Vitek 2 and Microscan Walkaway. This research aims to
analyze the suitability of bacterial identification and test antibiotic sensitivity of Microscan Walkaway device
from Beckman Coulter and Vitek 2 device from Biomerieux as a gold standard. The research conducted
was an observational cross-sectional study. Sampling was done consecutively. The study sample consisted
of 202 samples obtained from the results of positive isolates, during January-July 2019 at Dr. Soetomo
hospital. Positive isolates were examined for bacterial identification and antibiotic sensitivity using Vitek
2 and Microscan Walkaway. The results were statistically analyzed using SPSS. Examination of bacterial
identification using the Microscan device showed 34.2% of Gram-positive bacteria and 65.8% of Gramnegative bacteria, whereas with Vitek 2, results showed 34.7% of Gram-positive bacteria and 65.3% of
Gram-negative bacteria. Both of these instruments showed identification accuracy of 98.56% for Gram
positive bacteria and 100% for Gram negative bacteria with Kappa value: 0.814 and p <0.0001. The results
of the accuracy test for antibiotic sensitivity of multidrug resistance bacteria showed compatibility with p
value <0,0001. There is very good agreement between Vitek 2 and Microscan Walkaway in the identification
of bacteria and antibiotic sensitivity.

Author Biographies

Ria Pingkan Ganda

Resident, Clinical Pathology Specialist Programme, Clinical Pathology Department, Faculty of Medicine,
Universitas Airlangga - Dr. Soetomo Hospital, Surabaya, Indonesia

Aryati

Lecturer, Clinical Pathology Department,
Faculty of Medicine, Universitas Airlangga - Dr. Soetomo Hospital, Surabaya, Indonesia

Sidarti Soehita

Lecturer, Clinical Pathology Department,
Faculty of Medicine, Universitas Airlangga - Dr. Soetomo Hospital, Surabaya, Indonesia

Published

2021-03-24

How to Cite

Ria Pingkan Ganda, Aryati, & Sidarti Soehita. (2021). Suitability Tests for Bacterial Identification and Antibiotic Sensitivity Tests using Microscan Walkaway on Vitek 2. Indian Journal of Forensic Medicine & Toxicology, 15(2), 3985-3991. https://doi.org/10.37506/ijfmt.v15i2.14995