Suitability Tests for Bacterial Identification and Antibiotic Sensitivity Tests using Microscan Walkaway on Vitek 2
Keywords:Identification of bacteria, antibiotic sensitivity, Microscan WalkAway, Vitek 2
Culture or bacterial culture is the gold standard for diagnostic examination to detect the presence of
microorganisms in the patient’s body. The development of culture technology and culture-based automatic
devices for diagnostic examination are widely researched. At present, there are several types of automatic
blood culture instruments in Indonesia, namely Vitek 2 and Microscan Walkaway. This research aims to
analyze the suitability of bacterial identification and test antibiotic sensitivity of Microscan Walkaway device
from Beckman Coulter and Vitek 2 device from Biomerieux as a gold standard. The research conducted
was an observational cross-sectional study. Sampling was done consecutively. The study sample consisted
of 202 samples obtained from the results of positive isolates, during January-July 2019 at Dr. Soetomo
hospital. Positive isolates were examined for bacterial identification and antibiotic sensitivity using Vitek
2 and Microscan Walkaway. The results were statistically analyzed using SPSS. Examination of bacterial
identification using the Microscan device showed 34.2% of Gram-positive bacteria and 65.8% of Gramnegative bacteria, whereas with Vitek 2, results showed 34.7% of Gram-positive bacteria and 65.3% of
Gram-negative bacteria. Both of these instruments showed identification accuracy of 98.56% for Gram
positive bacteria and 100% for Gram negative bacteria with Kappa value: 0.814 and p <0.0001. The results
of the accuracy test for antibiotic sensitivity of multidrug resistance bacteria showed compatibility with p
value <0,0001. There is very good agreement between Vitek 2 and Microscan Walkaway in the identification
of bacteria and antibiotic sensitivity.
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