Systemic Effects of Occlusal Disharmony on Corticosterone Serum Levels in Rattus Norvegicus


  • Suhartini
  • Endang Joewarini
  • Ida Bagus Narmada



Occlusal disharmony, Corticosterone, Stress, Trauma occlusion, Temporomandibular disorders


Background: Mastication process occurs over time and it can lead to occlusal reduction of the teeth. This
causes reducing occlusal contact of teeth and it will lead to disharmony occlusion disorders. The occlusion
disharmony is recognized by the body as a stressor that systemically affects the homeostasis in the body,
especially the production of hormones.
Objective: The aim of this study was to determine the systemic effect of occlusal disharmony on corticosterone
serum levels in Rattus norvegicus.
Method: The research method of this study used experimental design and conducted among 7 male Rattus
Norvegicus in each groups. In intervention group, an occlusal reduction was ± 1 mm in all molars and
for control group did not given any intervention. Moreover, at 1st day, 7th day, 14th day and 21st day was
taken 2 cc of blood via infraorbital vein. The measurement of Corticosterone levels used Enzyme Linked
Immunosorbent Assay (ELISA).
Result: Anova test results showed that corticosterone levels was significant difference between each groups
(p <0.05). Tukey HSD test showed there was a significant difference between intervention group and control
group on 1st day, in intervention group on 7th day (p <0.05).
Conclusion. The results of this study showed that there were differences of corticosterone serum levels
among mice who experienced occlusion disharmony so it concluded that the dental occlusion disharmony
had a systemic impact to corticosterone serum levels in Rattus norvegicus

Author Biographies


Doctoral Student. Doctoral Program of Medical Science, Faculty of Medicine, Airlangga University,

Assistant Professor,Department of Biomedic, Faculty of Dentistry, University of Jember, Indonesia

Endang Joewarini

Professsor, Department of Anatomy Patology, Faculty of Medicine, Airlangga University, Indonesia

Ida Bagus Narmada

Professsor,Department of Orthodontics, Faculty of Dental Medicine, Airlangga University, Indonesia



How to Cite

Suhartini, Endang Joewarini, & Ida Bagus Narmada. (2021). Systemic Effects of Occlusal Disharmony on Corticosterone Serum Levels in Rattus Norvegicus. Indian Journal of Forensic Medicine & Toxicology, 15(2), 4046-4049.