Transferring of Heavy Metal Co-Resistance and Antibiotics Resistance of Bacteria Isolated from Sewage Water
Keywords:Conjugation, K. pneumonia, antibiotic, nickel, zinc, and sewage water.
Fifty-five samples of water have been collected from Baghdad sewage stations in the period between 9th
till 18th of Jul in 2018. Forty-two Klebsiella pneumonia isolates have been identified from water samples
according to its morphological, cultural, and chemical characteristics. The susceptibility of the isolated
bacteria to antibiotic were performed against ten types of antibiotics representing most common antibiotic
categories, the results showed differences in the bacterial susceptibilities against the currently antibiotics
ranged according to the antibiotic itself. The results were ranging from medium to low of bacterial resistance.
In addition four of the bacterial isolates were multidrug resistant bacteria (MDR). The results of bacterial
abilities to tolerate the toxicities of nickel and zinc showed high levels of the metal tolerate. Plasmid profiling
of bacterial isolates were performed, and then plasmids curing processes were done to delete the bacterial
plasmid and then both antibiotic resistance and heavy metal tolerance of cured isolates were performed to
determine the origin of resistance and tolerance encoded gene. Nor resistance or tolerance patterns of cured
bacteria showed difference when compared with non-cured (original) isolates such as have been showed
from results. The conjugation results of four Klebsiella pneumonia isolates didn’t transfer metal tolerance
genes between two successfully conjugated bacteria, while some of resistance genes transfer conjugationally
from donor to recipient cells.
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