Molecular Investigation of Quinolone Resistant Genes among Clinical Psedomonas aeruginosa Isolates
Keywords:Fluoroquinolone, gyrA,gyrB. aeruginosa, 16s rRNA, antibiotic resistance.
The current research was performed in Al-imam AL- Sadiq Hospital, Hilla Teaching Hospital, Babylon
city, Iraq to investigate the resistance gene of fluoroquinolones (FQs) in P. aeruginosa clinical isolates
of taken from different origins. Mutations arising at the bacterial gyrA, gyrB, parC, par E gene belong to
DNA gyrase and topoisomerase IV are the fluoroquinolone tolerance essential mechanism in P. aeruginosa..
and over expression of efflux pumps . (80) clinical isolates collected by Transport swabs from various
source [(burn 45(56.25 %), wound 15 (18.75 %), ear 3 (3.75 %), blood 2 (2.5 %), urine 4(5 %),sputum
11(13.75)] that initially identified by culturing on MacConkey agar, blood agar and cetrimide agar selective
media for Pseudomonas aeruginosa, P. aeruginosa chromogenic agar then diagnosed by performing some
morphological and biochemical tests. The second diagnosis to (10) isolates was done by VITEK 2 Compact
system using VITEK 2GN,and by using PCR technique for genotyping detection by the 16s rRNA gene.
A susceptibility test was applied to all (80) isolates using 9 antibiotics through resorting to Kirby-Pour
method. The results of this test showed that isolates which represented the highest resistance to ciprofloxacin
(71%),levofloxacin(75%) , aztreonam(87.5%), but isolates showed low resistance to impenem 18.75% and
0%resistant to meropenem. (gyrA, gyrB) subunit of DNA gyrase is target of flouroquinolon antibiotic has
been screened by PCR reaction. The results showed that these genes present in P. aeruginosa at (100 percent,
100 percent,), respectively..
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