Antibacterial Activity of Eucalyptus Camaldulensis Extracts on Staphylococcus aureus Isolated from Nasal Cavity

Authors

  • Zahraa Sedeeq Qasim
  • Farah Hazem Omer
  • Maimonah Qasim Yahya

DOI:

https://doi.org/10.37506/ijfmt.v15i2.15039

Keywords:

Eucalyptus camaldulensis, Staphylococcus aureus, Virulence factors.

Abstract

To study the effect of prepared Eucalyptus camaldulensis in aqueous, ethanol and flavonoid extracts by
conducting susceptibility tests against Staphylococcus aureus isolated from nasal carrier of healthy subjects,
in addition to detection some of virulence factors by conducting ?-lactamase enzyme test, and biofilm
formation test. This study included 250 healthy subjects, the males were 125(50%) and 125(50%) females.
Two swab samples were collected from each subject, the studied samples collected during one year period
from (April / 2018- April 2019). Different culture media and biochemical tests were used for isolation
and identification the isolates as Staphyllococcus aureus, in addition to prepared three types of Eucalyptus
camaldulensis extracts in form of aqueous, ethanol and flavonoid. Susceptibility test was conduct by disc
diffusion method, then study some of virulence factors of Staphyllococcus aureus by conducting ?-lactamase
enzyme test and biofilm formation test. Among 250 healthy subjects involved in this, study Staphyllococcus
aureus 68 (27%) isolates were detected, isolates from male was 40 (59%), while from female was 28 (41%),
only 39 (57 %) isolates were sensitive to different concentrations of Eucalyptus camaldulensis, of both
aqueous and ethanol extracts, ethanol extract had a higher effect on Staphyllococcus aureus than aqueous
extract, while 45 (66%) isolates were sensitive to flavonoid extract. Twelve (18%) isolates form a biofilm in
tube method, and 48 (71%) isolates showed ?-lactamase test positive. A higher nasal carriage were in males
in this study than females. The anti Staphyllococcus aureus effect of Eucalyptus camaldulensis extracts
were flavonoid followed by ethanol then aqueous extracts, some of Staphyllococcus aureus isolates were
highly virulence by showing a positive result for ?- lactamase enzyme and biofilm formation test. this study
shown that the flavonoid extract of E. camaldulensis have a great potential as antimicrobial agents on S.
aureus than crude aqueous and ethanol extracts, many nasal pharmaceutical preparation can prepare from
E. camaldulensis in crude and flavonoid form for treatment infections and reduce nasal carrier of S. aureus.

Author Biographies

Zahraa Sedeeq Qasim

Assistant Lecturer

Farah Hazem Omer

Lecturer at University of Mosul, College of Pharmacy, Department of Clinical Laboratory
Science Mosul, Iraq

Maimonah Qasim Yahya

Lecturer at University of Mosul, College of Pharmacy, Department of Clinical Laboratory
Science Mosul, Iraq

Published

2021-03-24

How to Cite

Zahraa Sedeeq Qasim, Farah Hazem Omer, & Maimonah Qasim Yahya. (2021). Antibacterial Activity of Eucalyptus Camaldulensis Extracts on Staphylococcus aureus Isolated from Nasal Cavity. Indian Journal of Forensic Medicine & Toxicology, 15(2), 4260-4266. https://doi.org/10.37506/ijfmt.v15i2.15039