Genotype Frequency of Cytochrome P450 2C9 (CYP2C9) Mutations in Iraqi Ethnic Groups

Authors

  • Suhad R. AL- Tayie
  • Salwa Jaber Al-Awadi
  • Iqbal harbi Mohammed Al-Zaidi

DOI:

https://doi.org/10.37506/ijfmt.v15i2.15045

Keywords:

Cytochrome P450 2C9 ; SNP , polymorphism ; population

Abstract

In Middle East, There is a little knowledge about the cytochrome P450s genotyping and there has not found
any investigation about the CYP2C9 allelic diversity in Iraqi population nowadays .CYP2C9 genotypes were
established in a total of 400 subjects of healthy Iraqis volunteers from different areas in Iraq (Arab ,Kurd
and Turkmen). After extraction of DNA from blood cells and polymerase chain reaction, SNP genotyping
for CYP2C9 variants (2,3,4 and 5) was performed by using the primer extension KASP® genotyping assay.
The agreement with “Hardy-Weinberg equilibrium” for detected the distribution of CYP2C9 genotype
was proved by (X2 – test) .The CYP2C9*2 , CYP2C9*3 and CYP2C9*5 alleles variant were identified in
Iraqi population , whereas the CYP2C9*4 was absent , The results revealed that there was non- significant
association observed in all CYP2C9 genotypes among Iraqi ethnic groups .then The CYP2C9 genotypes
results in our study were compared to other populations and The results exhibited that the frequency of Iraqi
population CYP2C9 variants were comparable with other countries and ethnic groups. This is the first report
of CYP2C9 variants from Iraq; the results are assist as a database on CYP2C9 gene polymorphisms and
clinical data baseline for dosing and avoiding adverse reactions of drugs metabolized by CYP2C9 in Iraqis
patients.

Author Biographies

Suhad R. AL- Tayie

Lecturere at College of Pharmacy /Ahel AL- Bait University

Salwa Jaber Al-Awadi

Professor at College of Biotechnology - Al-Nahrain
University

Iqbal harbi Mohammed Al-Zaidi

Lecturere at Research Department /Horticulture office/Ministry of Agriculture

Published

2021-03-24

How to Cite

Suhad R. AL- Tayie, Salwa Jaber Al-Awadi, & Iqbal harbi Mohammed Al-Zaidi. (2021). Genotype Frequency of Cytochrome P450 2C9 (CYP2C9) Mutations in Iraqi Ethnic Groups. Indian Journal of Forensic Medicine & Toxicology, 15(2), 4296-4301. https://doi.org/10.37506/ijfmt.v15i2.15045