Effect of Nigella Sativa (Black Cumin), Vitamin E and their Combination on Metoprolol Induced Hepatotoxicity in Rabbits
Keywords:Metoprolol, hepatotoxicity, Nigella Sativa, vitamin E, antioxidants, hepatoprotective agents, interleukins.
Background: Metoprolol (MET) is a selective Beta-1 blocker. It is metabolized in the liver. Unlike most
Betablockers, Metoprolol is unique in causing liver toxicity, which is infrequent, ranging from raised liver
enzymes to severe liver damage. Aim: hepatotoxicity was induced in rabbits, and protective effects of
Nigella sativa (NS), Vitamin E (Vit E), and a combination of Nigella sativa + Vitamin E were investigated
on Metoprolol-induced hepatotoxicity.
Methods: 36 male rabbits were divided into six groups and treated as follow: Metoprolol (20mg/kg),
Nigella sativa (2ml/kg), control group, Metoprolol + Nigella sativa, Metoprolol + Vitamin E ( 175mg/
kg/day), Metoprolol + Nigella sativa + Vitamin E. Treatments were given orally for two weeks. Liver
enzymes, malondialdehyde, glutathione, and interleukins (IL-6, IL-10) were measured in serum and liver
homogenates. Histopathological examinations were done for two animals in each group.
Results: Elevated liver enzymes and histopathological changes were seen in all Metoprolol-treated animals.
Nigella sativa, Vitamin E, and Nigella sativa + Vitamin E reduced Metoprolol-induced rise in liver enzymes.
Metoprolol increased malondialdehyde concentration in serum and liver homogenate. Treatment with
Nigella sativa, Nigella sativa + Vitamin E insignificantly decreased these levels. Neither Metoprolol nor
Metoprolol and Nigella sativa, Vitamin E, and their combination changed the levels of IL-6 or IL-10.
Conclusion: Metoprolol increased liver enzymes, and treatment with Nigella sativa, Vitamin E, and Nigella
sativa + Vitamin E significantly modified rise in liver enzymes. Metoprolol and other treatments had little
effect on IL-6 or IL-10.
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