Effect of Nigella Sativa (Black Cumin), Vitamin E and their Combination on Metoprolol Induced Hepatotoxicity in Rabbits

Authors

  • Fatima Hussein
  • Jawad Ahmed

DOI:

https://doi.org/10.37506/ijfmt.v15i3.15457

Keywords:

Metoprolol, hepatotoxicity, Nigella Sativa, vitamin E, antioxidants, hepatoprotective agents, interleukins.

Abstract

Background: Metoprolol (MET) is a selective Beta-1 blocker. It is metabolized in the liver. Unlike most
Betablockers, Metoprolol is unique in causing liver toxicity, which is infrequent, ranging from raised liver
enzymes to severe liver damage. Aim: hepatotoxicity was induced in rabbits, and protective effects of
Nigella sativa (NS), Vitamin E (Vit E), and a combination of Nigella sativa + Vitamin E were investigated
on Metoprolol-induced hepatotoxicity.
Methods: 36 male rabbits were divided into six groups and treated as follow: Metoprolol (20mg/kg),
Nigella sativa (2ml/kg), control group, Metoprolol + Nigella sativa, Metoprolol + Vitamin E ( 175mg/
kg/day), Metoprolol + Nigella sativa + Vitamin E. Treatments were given orally for two weeks. Liver
enzymes, malondialdehyde, glutathione, and interleukins (IL-6, IL-10) were measured in serum and liver
homogenates. Histopathological examinations were done for two animals in each group.
Results: Elevated liver enzymes and histopathological changes were seen in all Metoprolol-treated animals.
Nigella sativa, Vitamin E, and Nigella sativa + Vitamin E reduced Metoprolol-induced rise in liver enzymes.
Metoprolol increased malondialdehyde concentration in serum and liver homogenate. Treatment with
Nigella sativa, Nigella sativa + Vitamin E insignificantly decreased these levels. Neither Metoprolol nor
Metoprolol and Nigella sativa, Vitamin E, and their combination changed the levels of IL-6 or IL-10.
Conclusion: Metoprolol increased liver enzymes, and treatment with Nigella sativa, Vitamin E, and Nigella
sativa + Vitamin E significantly modified rise in liver enzymes. Metoprolol and other treatments had little
effect on IL-6 or IL-10.

Author Biographies

Fatima Hussein

Post Graduate Ministry of Health, Basrah Health Directorate, Iraq

Jawad Ahmed

Assistant Professor, Department of Pharmacology, College of Medicine, University of Basrah, Iraq

Published

2021-05-17

How to Cite

Hussein, F. ., & Ahmed, J. . (2021). Effect of Nigella Sativa (Black Cumin), Vitamin E and their Combination on Metoprolol Induced Hepatotoxicity in Rabbits. Indian Journal of Forensic Medicine & Toxicology, 15(3), 1059-1065. https://doi.org/10.37506/ijfmt.v15i3.15457