Phytochemical Study and Isolation of Scopolutin from Pimpinella anisum Seeds Grown in Iraq

Authors

  • Noor Mohsen Nasser
  • Widad M K Al-ani
  • Zahraa A.E Al Naqqash

DOI:

https://doi.org/10.37506/ijfmt.v15i3.15462

Keywords:

Pimpinella anisum, GC/MS, Phytochemical screening, scopoletin.

Abstract

As widely known plants are divided into many categories, one of them is the herbs; which have been always
used as effective remedies to treat many health issues. One of these remedies belongs to our plant under
study Pimpinella anisum (anise), which belongs to Umbelliferae family, that has been characterized as an
aromatic plant used in traditional medicine as carminative, disinfectant, and galactagogue. Anise dry seeds
were collected from Iraqi herbal market to investigate their chemical constituents by using two methods:
one of them hydrodistillation method using Clevenger apparatus in which oil sample was collected and then
analyzed by GC/MS and revealed the presence of anethol as the major constituent with percentage of 82.91
% of the oil; the second method was alcoholic extraction by Soxhlet apparatus with ethanol till exhaustion
then the extract was concentrated by evaporation under vacuum, after that partition with ethyl acetate was
done, the ethanol layer was discarded while the ethyl acetate layer was concentrated under vacuum, the
collected fraction were analyzed by TLC comparing it with standard Scopoletin .Phytochemical screening
proved that the plant contain Scopoletin and was confirmed by comparing Rf value of the sample with the
standard in three different solvent systems.

Author Biographies

Noor Mohsen Nasser

Assistant Lecturer, Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, Mustansiriyah University, Baghdad, Iraq

Widad M K Al-ani

Assistant Professor, Department of Pharmacognosy and Medicinal Plants, College
of Pharmacy, Mustansiriyah University, Baghdad, Iraq

Zahraa A.E Al Naqqash

Lecturer/ Department of Pharmacognosy and Medicinal Plants, College of Pharmacy, Mustansiriyah University, Baghdad, Iraq

Published

2021-05-17

How to Cite

Nasser, N. M. ., Al-ani, W. M. K. ., & Naqqash, Z. A. A. . (2021). Phytochemical Study and Isolation of Scopolutin from Pimpinella anisum Seeds Grown in Iraq. Indian Journal of Forensic Medicine & Toxicology, 15(3), 1117-1122. https://doi.org/10.37506/ijfmt.v15i3.15462