The Effect of Aqueous Extract of Quercus infectoria Plant on Pap E gene Expression of Uropathogenic Escherichia coli
Keywords:uropathogenic Escherichia coli, Pap E gene, Quercus infectoria
One hundred twelve urine samples were collected from Baghdad hospitals and examined by different
identification techniques. Seventy isolates (62.5%) were diagnosed as Escherichia coli after microscopic
and cultural identifications. The result of PCR product electrophoresis on the isolates showed that thirteen
isolates (18.57%) have Pap E gene which are uropathogenic E. coli. Antibiotic susceptibility test was done,
and four high resistant strains were mixed with aqueous extract of Quercus infectoria plant in 96 well ELISA
plate and incubated for different times. After 0, 6, and 12 hr. of incubation, the effect of the plant extract on
the bacterial growth was determined by ELISA reader, and the effect on the expression of Pap E gene was
examined by real-time PCR. The results were showed that the higher effect of the extract was on the E10
strain growth that dropped from 3.184 at zero time of incubation to 2.378 and 2.281 after 6, and 12 hrs. of
incubation respectively. Also, shown a downregulation in the expression of Pap E gene of the isolates at
different times of incubation. The E10 strain shows decrease in the fold from 1 before the treatment with the
extract to 0.076 at zero time of incubation with the extract, after 6 and 12 hrs. of incubation it shows a total
down-regulation of the gene.
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