Purification and Characterization of Arginase from Serum of Healthy and Type II Diabetic Iraqi Patients

Authors

  • Noor N.O. Al-Saad
  • Fayhaa M. Khaleel
  • Essam F. A. Al-Jumaili

DOI:

https://doi.org/10.37506/ijfmt.v15i3.15475

Keywords:

Purifying arginase, DEAE-cellulose, Sepharose6B, diabetes mellitus.

Abstract

Arginase was purified from human serum of healthy and patient with type II Diabetes mellitus subjects.
The purification steps included, DEAE-cellulose chromatography and gel filtration on Sepharoe6B.The
molecular weight of native arginase was find to be (94 Kd), by gel filtration. The purified human serum
arginase is characterized by optimum pH of (9.5), optimum pH of stability is between (9-10), optimum
temperature (350C), temperature of stability is between (30-500C) in both healthy and patient subjects.

Author Biographies

  • Noor N.O. Al-Saad

    Lecturer, Bilad Al-Rafidain University College/ Medical Lab. Technologies Department

  • Fayhaa M. Khaleel

    Assist. Prof., Chemistry Dept. College of Science for Women, University of Baghdad, Baghdad, Iraq

  • Essam F. A. Al-Jumaili

    Prof., Genetic Engineering and Biotechnology Institute, University of Baghdad, Baghdad, Iraq

Downloads

Published

2021-05-17

Issue

Vol. 15 No. 3 (2021): Indian Journal of Forensic Medicine & Toxicology

Section

Articles

License

https://creativecommons.org/licenses/by-nc/2.0/deed.en

How to Cite

Purification and Characterization of Arginase from Serum of Healthy and Type II Diabetic Iraqi Patients. (2021). Indian Journal of Forensic Medicine & Toxicology, 15(3), 1194-1203. https://doi.org/10.37506/ijfmt.v15i3.15475