Purification and Characterization of Arginase from Serum of Healthy and Type II Diabetic Iraqi Patients

Authors

  • Noor N.O. Al-Saad
  • Fayhaa M. Khaleel
  • Essam F. A. Al-Jumaili

DOI:

https://doi.org/10.37506/ijfmt.v15i3.15475

Keywords:

Purifying arginase, DEAE-cellulose, Sepharose6B, diabetes mellitus.

Abstract

Arginase was purified from human serum of healthy and patient with type II Diabetes mellitus subjects.
The purification steps included, DEAE-cellulose chromatography and gel filtration on Sepharoe6B.The
molecular weight of native arginase was find to be (94 Kd), by gel filtration. The purified human serum
arginase is characterized by optimum pH of (9.5), optimum pH of stability is between (9-10), optimum
temperature (350C), temperature of stability is between (30-500C) in both healthy and patient subjects.

Author Biographies

Noor N.O. Al-Saad

Lecturer, Bilad Al-Rafidain University College/ Medical Lab. Technologies Department

Fayhaa M. Khaleel

Assist. Prof., Chemistry Dept. College of Science for Women, University of Baghdad, Baghdad, Iraq

Essam F. A. Al-Jumaili

Prof., Genetic Engineering and Biotechnology Institute, University of Baghdad, Baghdad, Iraq

Published

2021-05-17

How to Cite

Al-Saad, N. N. ., Khaleel, F. M. ., & Al-Jumaili, E. F. A. . (2021). Purification and Characterization of Arginase from Serum of Healthy and Type II Diabetic Iraqi Patients. Indian Journal of Forensic Medicine & Toxicology, 15(3), 1194-1203. https://doi.org/10.37506/ijfmt.v15i3.15475