Rapid Induction of Mesenchymal Stem Cells to Neural Cells
Keywords:mesenchymal stem cells, umbilical cord blood, neuronal induction, in vitro.
Background:-Human umbilical cord blood- mesenchymal stem cells(HUCB-MSCs) hold great promise in
vitro neural differentiation and therapy for neurodegenerative disorders. It has demonstrated that the number
and differentiating potential of bone marrow mesenchymal stem cells (MSCs) decrease with age. Therefore,
the search for alternative sources of MSCs is of significant value.
Objective:-To determine the possibility of obtaining clonally expanded UCB-MSCs and evaluate their rapid
induction and differentiation into neural cells in vitro.
Methods:-MSCs were isolated from HUCB by combining gradient density centrifugation with plastic
adherence. In order to identify factors that able to lead to neural differentiation, the cultured cells were
treated with retinoic acid (RA) and B-mercaptoethanol (BME). Differentiating characterization of UCBMSCs
were detected by immunocytochemistry analysis.
Results:-UCB-MSCs appeared like the fibroblast cell and these cells were extensively expanded in culture.
The results showed that UCB-MSCs were positive for CD71and CD90 but were negative for CD34. The
immunocytochemistry staining indicated that the differentiated cells give positive response for nestine
marker. The result confirmed for neural progenitor differentiation of MSCs.
Conclusion: The results of this study confirmed that UCB provides a great source of stem cells for using in
treatment of neurodegenerative disorders of the central nervous system.
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