Entamoeba Histolytica, Identification in Asymptomatic Infection
Keywords:Entamoeba histolytica, Microscopic diagnosis, RT-PCR, Amebiasis, Carrier
Reliable detection the etiological agent of amoebic dysentery and extra-intestinal amoebiasis have Public
health importance specially in asymptomatic individual .The objective of the study includes two aspects,
the first to give correct perception of infection rate in asymptomatic individuals and the second aspect about
detection and diagnosis of the pathogenic species of Entamoeba histolytica from another morphologically
similar and commensal one using the molecular technique in stool samples of asymptomatic individuals.
During the study period from the beginning of April to the end of Jun /2018 , a total of 95 stool sample
was collected from asymptomatic 71 male and 24 female ages between 25-55 years old. Microscopic and
molecular diagnosis for E.histolytica was done using wet mount method and Real Time polymerase chain
reaction employing 18S rRNA gene
Respectively . The result highlighted the microscopic diagnosis of cyst stage in 33 asymptomatic patient
out of 95 (34.73%) with significant differences P<0.05 between males 22/71(66.66%) and females 11/24
(33.33%).On the other hand the molecular diagnosis results showed presence of Entamoeba histolytica in 19
sample out of 33 (63.33%) with significant differences P<0.05 between males 15/22 (68.18%) and females
4/11 (36.36%).Conclusion, The microscopic examination of stool sample show a possibility of error to
differentiate pathogenic E. histolytica from those of morphologically similar non-pathogenic species lead to
inaccurate results therefore, the molecular methods must be adopted for diagnosis. There is also a sign that
the pollution problem is still not controlled and is a risk factor for infection transmitted.
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