Forensic Assessment of Genotoxic Effects of Lead Metal on leaf Phragmites australis Using Random Amplified Polymorphic DNA (RAPD-PCR) Markers

Authors

  • Ahmed Ghalib Ibraheem
  • Reyam Naji Ajmi

DOI:

https://doi.org/10.37506/ijfmt.v15i3.15520

Keywords:

Forensic environmental, RAPD-PCR, Lead , Phragmites australis.

Abstract

The influence lead metal on DNA integrity in leaf plant can measure by potential effects of pollutants
accumulation metal, Pb in leaf plant Phragmites australis was subjected to RAPD analysis, have been found
metal at concentrations of S1, S2,S3 (0.045 , 2.97, 0.93) Respectively. polymorphisms became evident as
the presence and absence of DNA fragments in affected samples compared to effect samples. Five prime
was selected OP-L05, OP-M14, OP-M20, OP-V09 and OP-M05 were used , the genomic DNA of leaf did
not appear polymorphic band at OP-L05 except molecular weight 600bp and presence of polymorphic band
,OP-M20 show only polymorphic band at 850bp,OP-M05 did not show any polymorphisms and OP-M14,
OP-V09 primers appeared polymorphisms when compare unexposed in control, this study confirmed RAPD
markers a fast and simple technique can be used detection of forensic environmental.

Author Biographies

Ahmed Ghalib Ibraheem

Post graduate, Department of Biology Science, Mustansaryah University, POX 46079, Iraq-Baghdad

Reyam Naji Ajmi

Prof. , Department of Biology Science, Mustansaryah University, POX 46079, Iraq-Baghdad

Published

2021-05-17

How to Cite

Ibraheem, A. G. ., & Ajmi, R. N. . (2021). Forensic Assessment of Genotoxic Effects of Lead Metal on leaf Phragmites australis Using Random Amplified Polymorphic DNA (RAPD-PCR) Markers. Indian Journal of Forensic Medicine & Toxicology, 15(3), 1515-1523. https://doi.org/10.37506/ijfmt.v15i3.15520

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