Forensic Assessment of Genotoxic Effects of Lead Metal on leaf Phragmites australis Using Random Amplified Polymorphic DNA (RAPD-PCR) Markers
Keywords:Forensic environmental, RAPD-PCR, Lead , Phragmites australis.
The influence lead metal on DNA integrity in leaf plant can measure by potential effects of pollutants
accumulation metal, Pb in leaf plant Phragmites australis was subjected to RAPD analysis, have been found
metal at concentrations of S1, S2,S3 (0.045 , 2.97, 0.93) Respectively. polymorphisms became evident as
the presence and absence of DNA fragments in affected samples compared to effect samples. Five prime
was selected OP-L05, OP-M14, OP-M20, OP-V09 and OP-M05 were used , the genomic DNA of leaf did
not appear polymorphic band at OP-L05 except molecular weight 600bp and presence of polymorphic band
,OP-M20 show only polymorphic band at 850bp,OP-M05 did not show any polymorphisms and OP-M14,
OP-V09 primers appeared polymorphisms when compare unexposed in control, this study confirmed RAPD
markers a fast and simple technique can be used detection of forensic environmental.
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Re use and mixing of content policy- We follow Creative Commons Licence Policy. We follow CC BY. Please refer below for all details
This license lets others distribute, remix, adapt, and build upon our work, even commercially, as long as they credit us for the original creation.
- The journal allows readers to read, download, copy, distribute, print, search, or link to the full texts of its articles and allow readers to use them for any other lawful purpose.
- The journal allows the author(s) to hold the copyright without restrictions.
- The journal allows the author(s) to retain publishing rights without restrictions