Molecular Detection of Burkholderia cepacia and Comparative Study Between Medical Plant and Antibiotics against Bacteria Isolates from Cystic Fibrosis
Keywords:cystic fibrosis, Mentha, Lemon peel extract, PCR, Vitek2 compact system, antibiotics.
Background: One of the major deaths causes in the patients with cystic fibrosis (CF) is the impaired lung
function. Lately, Burkholderia cepacia was considered as a significant opportunistic pathogen in such
patients. Since the late 1970s, due to its increased isolation from CF patients, its ability to spread infections
in the community of CF patients, its role in disrupting lung function, and its inherent resistance to multiple
antibiotics. Those various factors are making the Burkholderia cepacia isolation one of the significant tasks
in cystic fibrosis health-care environment.
Objective: To identify the method and the complete recA nucleotide sequence, and to compare the isolated
bacteria from CF by medicinal plants and antibiotics.
Material and Methods: Bacterial identification of CF patients was carried out by standard microbiological
methods and identification was carried out using biochemical tests Vitek2 compact system, and PCR
identification to confirm the identification and evaluate the antibacterial activity in Mentha leaves and lemon
peel extracts against pathogenic bacteria.
Results: Biochemical tests and gene expression confirmed that Burkholderia cepacia and Pseudomonas spp
strains were isolated from 50 sputum samples. These results indicate that Mentha leaves extract is better than
lemon peel extract, compares with antibiotics reveal the resistance of these bacteria.
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