Molecular Association of Sexually Transmitted Infections and Methylation Pattern of SNRPN Gene Promoter among Males with Abnormal Semen

Authors

  • Noor AL-Hamid
  • Abdul Kareem AL-Kazaz

DOI:

https://doi.org/10.37506/ijfmt.v15i3.15581

Keywords:

Sexually Transmitted Infections , SNRPN genes , Infertility in man , Abnormal Semens

Abstract

Infertility affects about 8-12% of couples worldwide, and male infertility accounts for a half of these
cases, therefore, thisstudy was undertaken to focus on some factors affecting male’s fertility such as DNA
methylation and genital tract infections by sexually transmitted diseases. The study groups included infertile
males as a patient group (n=63) in an age mean (32.28 ± 6.88 years) and fertile males as a control group
(n=13)in an age mean (34.07 ±6.52years).
Seminal fluid was collected from all males attending a clinical laboratory for routine semen analysis, seminal
fluid analysis was performed according to World Health Organization guidelines, all of the 63 infertile males
was suffering from reduced sperm concentration (9.42 ± 8.70 million/ml), reduced progressive motility
(2.89 ± 5.45%) and abnormal sperm morphology (27.06 ± 16.50%), while control group have a higher
values thus, there was a highly significant difference in semen parameters between the two groups.
The DNA samples of the patients group (n=44) and control group (n=13) was subjected to bisulfite treatment
to convert un-methylated C-residues to uracil, this step was performed to allow the qualitative detection of
methylation at the promoter of Small nuclear ribonucleoprotein polypeptide N gene by Real time polymerase
chain reaction using two sets of designed primers (methylated and un-methylated sets) allowing one set of
the primers to anneal, and amplify the desired region.
The results of real time PCR for the detection of methylation in SNRPN gene promoter showed hyper
methylation in the promoter of SNRPN in some samples (22.7%), a slight methylation in others (20.4%) and
no methylation in other samples (56.8%) from infertile males, while all the 13 control samples showed no
methylation.

Author Biographies

Noor AL-Hamid


Assistant Professor, Department of Biotechnology University of Baghdad

Abdul Kareem AL-Kazaz

Professor and Dean of College of
Science, University of Baghdad

Published

2021-05-17

How to Cite

Noor AL-Hamid, & Abdul Kareem AL-Kazaz. (2021). Molecular Association of Sexually Transmitted Infections and Methylation Pattern of SNRPN Gene Promoter among Males with Abnormal Semen. Indian Journal of Forensic Medicine & Toxicology, 15(3), 1830-1836. https://doi.org/10.37506/ijfmt.v15i3.15581