The Adverse Effect of Air Pollution with Polycyclic Aromatic hydrocarbon (PAH) on 8-OXO-DG and gene expression (HOGG1) in Midland Refineries Company-Daura Refinery Workers
Keywords:Polycyclic aromatic hydrocarbon; Oxidative DNA damage; HOGG1, 8-oxoguanine
Introduction: Polycyclic aromatic hydrocarbons (PAHs) are a group of different kind of hazardous organic
chemicals which are considered to be the top of pollutants that released by petroleum industries exploration
activities to the environment. PAH Metabolites that affect initiation of cancer by reaction with DNA are
practically modified chemically by enzymes. PAH Mutagenic metabolites include radical PAH cations, diol
epoxides, and quinones. Reactive oxygen species (ROS) mostly produce by routine oxidation processes in
mitochondria is responsible for many types of DNA damage. The aim of study identify the DNA alteration
due to air pollution by modulation of gene expression and regulatory gene Human 8-oxoguanine DNA
Glycosylase (HOOG1) and association with 7,8-dihydro-8-oxoguanine (8-oxo-dg). Methods: there were
168 participants included in this study divided in three groups (control, office workers and in field workers)
.PAH, 8-OXO-DG and gene expression (HOGG1) detected for each participant by GC/MS, competitive
ELISA and quantitative-competitive reverse transcription-PCR respectively. Results: PAH were not detected
in the blood of control group, and there was significant difference in the concentration of PAH between
office and field workers. The concentration of (8-oxo DG) and (hOGG1) was significantly higher in field
worker than in office worker and control group, significant difference was found between office workers
and control. Discussion: It is observed that increased levels of PAH and its metabolites when exposed
to polluted air. PAHs metabolism is associated with production of ROS as well as oxidative damaged.
Among this damage, the most common lesion is (8-OHdG) .Increase in (8-OHdG) levels associated with
increase oxidative stress and (HOGG1) gene. Conclusion: (a) increased PAH and its metabolites levels in
serum causing high 8-OHdG and (HOGG1) expression levels in refineries workers (b) there is a correlation
between 8-OHdG and hOGG1 expression with PAH.
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