Determination of Antioxidant Compounds, Antibacterial Activity and Minerals Content of Broccoli
Keywords:Broccoli, Antioxidant activity, Antibacterial, Minerals
The aim of this study was to examine the antioxidant compounds, antibacterial activity and minerals content
of broccoli stem and floret. The antioxidant activity was determined by measuring total phenolic content
(TPC) and 2,2-diphenyl-1-picrylhydrazyl (DPPH). The antibacterial activity was determined using paper
disc method against two bacteria namely Klebsiella sp, Escherichia coli, Staphylococcus aureus and P.
aeruginosa. Minerals concentrations of broccoli stem and floret were determined using atomic absorption
spectrometry. The results showed that floret sample had significantly (P <0.05) higher total phenol content
and antioxidant activity compared to stem sample. The result showed that the acetone extracts of broccoli
stem and floret were effective against all the bacteria tested. The majority of the broccoli materials were rich
in some of the essential minerals like Na, K, Ca, Mg, Mn, Cu, Fe, and Zn, which are known to be beneficial
for health. In general, the concentrations of minerals in the broccoli stem and floret were detected. broccoli
floret higher minerals concentration of K, Ca, Mg, Mn, Cu, Fe, and Zn of floret than stem. Results showed
part of plant effect of the antioxidant compounds and minerals content. Results of the present study provide
vital data on the availability of some antioxidant compounds and essential minerals, which can be useful to
provide dietary information for designing value-added foods and for food bio fortification.
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