Partial Purification of Dipeptidyl Peptidase-4 From Human Blood Serum and Study the Inhibitory Effect of Some Nitrile Derivatives on it

Authors

  • Firas Shawqi Algburi
  • Nadia Y Al-Tikrity
  • Hasan Ulusal
  • Tugba Taskin-Tok

DOI:

https://doi.org/10.37506/ijfmt.v15i3.15755

Keywords:

Dipeptidyl peptidase-4, molecular docking, nitrile,gel filtration

Abstract

The research includes the partial purification of dipeptidyl peptidase-4(Dpp-4) from a person’s natural serum.
The enzyme was purified in three steps, precipitation by ammonium sulfate (50%), dialysis and gel filtration
by Sephadex G-100. The results showed a significant increase of Dpp-4 activity and specific activity,and a
decrease in total activity after purification.The descent of the purified enzyme with the highest activity from
the separation column was identical to the descending of the protein with the highest activity. The inhibitory
activity of nitrile derivatives that were used on the purified enzyme was studied. Nitrile derivatives were
used to inhibit the activity of the Dpp-4, selected based on similarity in sites of effective groups between
derivatives and anti-hyperglycemic drugs (Dpp-4 inhibitors type). The results show an inhibitory effect by
the two nitrile derivatives with different concentrations.These inhibitors can work to reduce the level of
blood sugar in people with diabetes. Moreover,Molecular docking was exerted to understand interaction
mechanism of the compounds against Dpp-4 at atomic level based on experimental processes.

Author Biographies

Firas Shawqi Algburi

Assistant Professor, Department of Chemistry,College of Science, Tikrit University, Iraq

Nadia Y Al-Tikrity

Dour Technical
Institute,Northern Technicial University, Iraq

Hasan Ulusal

Researcher, Department of Medical Biochemistry,Faculty of
Medicine, University of Gaziantep, Turkey

Tugba Taskin-Tok

Department of Chemistry, Gaziantep University, Gaziantep, Turkey

Published

2021-05-17

How to Cite

Firas Shawqi Algburi, Nadia Y Al-Tikrity, Hasan Ulusal, & Tugba Taskin-Tok. (2021). Partial Purification of Dipeptidyl Peptidase-4 From Human Blood Serum and Study the Inhibitory Effect of Some Nitrile Derivatives on it. Indian Journal of Forensic Medicine & Toxicology, 15(3), 2969-2976. https://doi.org/10.37506/ijfmt.v15i3.15755

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