The Effect of Black Cumin (Nigella sativa) Oil Supplementation on Hematotoxicity Induced by Benzene in Gasoline Vapors from Gas Stations in Male Wistar Rats
Keywords:Benzene, gasoline vapors from gas stations, black cumin, CYP2E1, GSH, MDA, hematological profile
This study aimed to prove the effect of black cumin oil supplementation through analysis of levels of
CYP2E1, MDA, GSH, and hematological profiles in experimental animals, Wistar rats, which were exposed
to benzene in gasoline vapors from gas stations. Sixty-four experimental Wistar rats were divided into eight
groups with eight rats each, two control groups (C1 and C2), and six experimental groups (T1, T2, T3, T4,
T5, and T6). Blood samples of experimental rats were taken through intracardiac with ketamine-xylazineacepromazine anesthesia on day 15 for group C1, C2, T1, T3, and T5; and day 29 for group T2, T4, and T6.
The ELISA method measured the levels of CYP2E1, GSH, and MDA in this study, while the hematological
profile used a hematology analyzer. This study indicates that benzene in gasoline vapors exposure caused
significant hematotoxicity (P <0.05). Supplementation of black cumin seed oil at a dose of 2.5 ml/kg, 5 ml/
kg, and 10 ml/kg, all of which have the potential to provide a protective effect against the hematotoxicity of
benzene in gasoline vapors through increased internal antioxidants (GSH). The GSH can play a role in the
mechanism of vapor metabolism of substances in gasoline vapors in the body through decreased CYP2E1
levels and decreased MDA levels. Further research is still needed with a more extended treatment duration
to ascertain the chronic effects of benzene hematotoxicity on the genomic pathway to assess black cumin’s
role, whether in the form of oil, powder, or other forms of extraction.
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Re use and mixing of content policy- We follow Creative Commons Licence Policy. We follow CC BY. Please refer below for all details
This license lets others distribute, remix, adapt, and build upon our work, even commercially, as long as they credit us for the original creation.
- The journal allows readers to read, download, copy, distribute, print, search, or link to the full texts of its articles and allow readers to use them for any other lawful purpose.
- The journal allows the author(s) to hold the copyright without restrictions.
- The journal allows the author(s) to retain publishing rights without restrictions