Genotyping and DNA Sequencing of Toxoplasma gondii from Women and Domestic Chickens in the Wasit Province Iraq

Abstract

The current study included two main axes, which are the investigation of the rates of toxoplasmosis in the
placenta in pregnant women who experience miscarriage and normal delivery, and the rate of infection in
the tissues of domestic chicken (brain, liver and muscles) in Wasit province. A total of 96 samples were
collected and subjected to PCR. The sample included 24 tissue samples collected from women of normal
childbirth or abortion, and 72 samples collected from local chickens, 24 for each of (brain, liver and muscles).
Results revealed that the difference between the infection rate in women (62.5%) and the infection rate in
the chickens (56.9%) was not significant. Also, the infection rates of Brain (62.5%), Liver (70.8%), and
Muscles(58.3%) of chicken were not significant.