Analysis of Capsicum annuum L. Methanolic Extract and Its Potential as a Hepatoprotector
Keywords:TLC, Immunohistochemistry, MAPK 1 expression, Hepatoprotector.
ThinLayerChromatography(TLC) method performed to purify capsaicin compound in C. annum L. and test the
bioactive component of Capsicum Annum L on MAPK1 target proteins using the Immunohistochemistry
(IHC) method to determine the reactions between receptor-ligand (antigen-antibody) complexes formed and
become a reference in vivo tests. Natural ingredients need to be extracted to get some or all of the active
ingredients used to synthesize the right dosage, easily stored, and maintain for a long time. The active
ingredient of capsaicin is efficacious as a stimulant of gastric acid secretion and prevents infections in the
digestive system.The immunohistochemical examination is intended to determine the expression of MAPK1
(ERK) on cells of mice hepatocytes (Mus musculus). MAPK 1 (ERK) expression score data were obtained
using the modified Remmele method is the result of multiplying the percentage score of immunoreactive
cells with the color intensity score on immunoreactive cells. The abnormal target protein signaling pathway
contributes to cell proliferation, differentiation, survival, and uncontrolled cell apoptosis.Analysis of amino
acid residues, Capsaicin to MAPK1haverelativelysimilarresiduessothatitcanbeconcludedthat the binding
sites of the two compounds are close to similarities and affect receptors at relatively similar sites, namely
oncogenicreceptors/ proteins. Concluded that the ethanol extract of Capsicum. annum L. had a greater in the
powder form , TLC method showed the capsaicin standard was 1020 µg/ml, that capsaicin in chilies (Capsicum
Annum L.) can synergistically inhibit cell through reactions in the anti-apoptotic MAPK 1 potentiates the
antiproliferative action that can promote hepatoprotector, a finding that demands further clinicaltesting.
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