Detection of Campylobacter jejuni and Campylobacter coli by a PCR assay in Patients with Diarrhea
Keywords:Campylobacter sp. ,PCR , hipO, glyA , diarrhea
Campylobacter sp. is complex taxonomy, fastidious growth criteria, and biochemical inertness, identification
of campylobacter in clinical samples by traditional cultures is problematic and slow. Because of that a rapid,
responsive, and precise polymerase chain reaction (PCR) assay capable of detecting and differentiating
Campylobacter jejuni (hipO) and Campylobacter coli (glyA) directly from human feces. Diagnostic PCR
on template DNA extracted directly from the stool offers catchy advantages including the reduced time of
analysis and detection of non-viable and non-cultivable bacteria contained in the sample.From a human
patient with diarrhea by using conventional PCR assay. One hundred fecal samples were collected from
patients suffering from watery, bloody, and mucous diarrhea, aged (1 month-60 years old) from both sexes
(55Male, 45 Female). From many places in Thi-Qar province, Bint Alhuda Hospital, and Outpatient Clinics
during the period from August 2020 to November 2020. Four isolates (4%) were diagnosed as genus C.coli
and one isolate (1%) was diagnosed as genus C.jejuniby using glyA and hipO gene for C.coli and C.jejuni
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