Effects of Monosodium Glutamate (MSG) on Neuron Damages in Hippocampus in Sprague-Dawley rats
Keywords:Monosodium glutamate (MSG), histology,hippocampus, neurotoxic effects, animal model
Introduction: Monosodium glutamate (MSG) is a flavor enhancer commonly used worldwide. Studies have
shown that high dose of MSG could act as neurotoxic or excitotoxic agent for neurons in the central nervous
system. The aim of this study was to determine the effect of MSG on neuron changes of hippocampusanimal
Materials and Methods: A total of 25 white male Sprague-Dawleyrats, aged 8-10 weeks,were dividedinto
5 groups (2 control groups (i.e., none and solvent group) and3 treatment groups that received2, 4and 6 mg/
gram MSG orallyfor 30 days). Afterfour weeks on treatment, all animals were sacrificed and the entire brain
tissueswere removed and immediately fixed in formalin for hematoxylin and eosin (H&E) staining.
Results:The percentage of damaged neurons in three Cornuammonisareas of hippocampus was higher in
animal supplemented with MSG compared to controls. At the highest MSG concentration (6 mg/gram),
52.1%, 55.2% and 66.0% of neurons from Cornuammonis 1, 2, and 3, respectively were damaged. The
percentage of neuron damages in hippocampus was in dose-dependent manner.
Conclusion: Our data suggested that high dose of MSG increased thehippocampus neuron damages in dosedependent
effect. This suggests the neurotoxicity effect of high dose of MSG.
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