Lactobacillus Rhamnosus GG Role in the Suppression of Candida Albicans Causing Candidiasis (Thrush)

Abstract

Introduction: It is important to distinguish infecting strains of Candida albicans because isolates of Candida
species vary widely, both in their infection-causing capacity and in their sensitivity to antifungal agents.
Material and method: Thus this study provides isolate candida and identification of isolates from the oral
cavity and test the antimicrobial activity of Lactobacillus rhamnosus GG and their supernatants on the
viability of Candida albicans isolates. The diagnosis was based on conventional methods, and genetic
diagnosis was confirmed by PLC1 gene (PhosphoLipase C) amplification, the design primers were done by
using the database of Bank of NCBI-Gene and online design, all isolates gives positive PCR products with
molecular weight 459bp,
Result: The results showed that Lactobacillus rhamnosus GG and their supernatants decreased the log
cycles growth of all Candida albicans isolates with average (1.7 -2.1) logarithmic cycle for live cells and
(1.28 -1.79) logarithmic cycle for Lactobacillus rhamnosus GG supernatants, Conclusion: Lactobacillus
rhamnosus GG may be a suitable and effective alternative to treatment oral candidiasis free from side effects.