Detection of toxin-associated genes in seven spa-types of Staphylococcus aureus in Iraq
Keywords:sea, pvl, tst, spa-type, Iraq, wound
Background: Staphylococci frequently cause opportunistic infections in patients with underlying disease,
such as those with prosthetic devices, surgical patients, and patients with diabetes or individuals undergoing
dialysis. Consequently, detecting the toxin-associated genes in different S. aureus strains, isolated from
patients with wound and burn injuries and their environments, is essential to monitor and prevent the
complication in such cases.
Methods: Identification ofS. aureus that isolated from burn and wound injuries has been done according
to the biochemical tests, then Spa-typing method was used to diagnose at the strain level. Toxin-associated
genes, enterotoxin gene(sea, seb),exfoliative gene(eta), toxic shock syndrome gene(tst), and pantonevalentine leukocidin(pvl), were detected by polymerase chain reaction (PCR) method.
Results: Two isolates were harboring tstand a single isolate harbored pvl, at the time that no isolates were
foundetaandsebpositive among 19 S.aureus. While sea was the predominant toxin-associate gene in the
studied S. aureus strains, it was detected in 13 isolates.
Conclusion: Distribution of specific spa-type/sea-positive in diabetic foot ulcers at hospitals, indicating the
poor sanitization conditions at Iraqi wound wards, and it is recommended to take swabs from the patients
and their environments as a routine procedure
How to Cite
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
Re use and mixing of content policy- We follow Creative Commons Licence Policy. We follow CC BY. Please refer below for all details
This license lets others distribute, remix, adapt, and build upon our work, even commercially, as long as they credit us for the original creation.
- The journal allows readers to read, download, copy, distribute, print, search, or link to the full texts of its articles and allow readers to use them for any other lawful purpose.
- The journal allows the author(s) to hold the copyright without restrictions.
- The journal allows the author(s) to retain publishing rights without restrictions