Antibacterial Activity of Methanol Extract of Red Dragon Fruit Peel ( Hylocereus polyrhizus) against MethicillinSusceptible Staphylococcus aureus ( MSSA) ATCC 25923 and Methicillin Resistant Staphylococcus aureus (MRSA) In Vitro

Authors

  • Shazia Hafazhah Aulia
  • Yuani Setiawati
  • Eko Budi Koendhori

DOI:

https://doi.org/10.37506/ijfmt.v15i3.15963

Keywords:

Antibacterial activity, Hylocereus polyrhizus, Methicillin-Resistant Staphylococcus aureus, Methicillin-Susceptible Staphylococcus aureus, Red dragon fruit peel

Abstract

Background: Staphylococcus aureus is one of the most virulent Staphylococcus which could cause systemic
broad-spectrum infections and developed resistant strains. Red dragon fruit (Hylocereus polyrhizus) peel
has a broad antibacterial activity against both gram-negative and positive bacteria, including Staphylococcus
aureus. Objective: To determine and compare the antibacterial activity of methanol extract of red dragon
fruit peel against Methicillin-Susceptible Staphylococcus aureus ATCC 25923 and Methicillin-Resistant
Staphylococcus aureus. Method: Well diffusion test method was performed on 5 concentrations: 100%,
75%, 50%, 25%, and 0%, dissolved in DMSO 10% where at a concentration of 100% contains 1g/mL
extract. The inhibition zone elicited as clear zone around the well then measured and analyzed with SPSS
using One-Way ANOVA and Post Hoc LSD, to determine the significance of each concentration. Results:
The mean diameter of the inhibition zone observed towards Methicillin-Susceptible Staphylococcus aureus
was 9.5333 ± 0.26822 - 15.4167 ± 0.22048, while bigger zone was found in Mueller-Hinton plate with
Methicillin-Resistant Staphylococcus aureus which is 12.30 ± 0.20817 - 17.80 ± 0.25166 mm. Conclusion:
This study showed that methanol extract of red dragon fruit peel has antibacterial activity against S. aureus,
and shows higher antibacterial activity against Methicillin-Resistant Staphylococcus aureus than MethicillinSusceptible Staphylococcus aureus.

Author Biographies

Shazia Hafazhah Aulia

Student, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Yuani Setiawati

Lecturer,Department of
Pharmacology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia,

Eko Budi Koendhori

Chief, Department of
Microbiology, Faculty of Medicine, Universitas Airlangga, Surabaya, Indonesia

Published

2021-05-17

How to Cite

Shazia Hafazhah Aulia, Yuani Setiawati, & Eko Budi Koendhori. (2021). Antibacterial Activity of Methanol Extract of Red Dragon Fruit Peel ( Hylocereus polyrhizus) against MethicillinSusceptible Staphylococcus aureus ( MSSA) ATCC 25923 and Methicillin Resistant Staphylococcus aureus (MRSA) In Vitro. Indian Journal of Forensic Medicine & Toxicology, 15(3), 4269-4273. https://doi.org/10.37506/ijfmt.v15i3.15963