Study the Reproductive Pattern of Different Breeds Bitches in Iraq by estimation of Kisspeptin and detection ovulation time.

Authors

  • Sulake Fadhil Abbas Al-Zubaidi
  • Souhayla O. Hussain

DOI:

https://doi.org/10.37506/ijfmt.v15i3.15974

Keywords:

Bitches, Progesterone, Ovulation, Kisspeptin, Seasonality.

Abstract

This study was conducted to investigate the reproductive pattern of bitches by estimation the optimum
time of ovulation and kisspeptin level. The study included 245 bitches (different breeds), from March/2019
to the end of February/2020.Canine ovulation timing kits was applied in order to determine the ovulation
time. For estimation of Kisspeptin, blood collection of 80 samples allocated approximately 7 samples for
each monthby using ELISA kit. The resultsrevealed the time of ovulation ranged from 2 to 5 days after the
beginning of the estrus phase.The results showed that concentration of kisspeptin during different phases of
the estrous cycle was (257.01±11.08, 262.93±9.15, 227.98±14.91 and 244.89±11.99) pg/mL for Proestrus,
Estrus, Diestrus, and Anestrus respectively with no significant (p>0.05) differences among different estrous
cycle phase. The concentration of kisspeptin in which distributed according to the season showed that it
was (218.26±11.92, 254.12±10.09, 246.00±10.22, and 287.11±9.00) pg/ml for Spring, Summer, Autumn,
and Winter, respectively and there was a significant difference (p<0.05) between Summer and Winter. As
a conclusion, the ovulation timing kit was accurate and useful for detecting the time of ovulation and the
season had a significant effect on the kisspeptin level.

Author Biographies

Sulake Fadhil Abbas Al-Zubaidi

PhD Student, Surgery and Obstetric Department, College of Veterinary Medicine / University of Al-Qasim Green /Iraq

Souhayla O. Hussain

Prof. Surgery and Obstetric Department, College of Veterinary Medicine University of Baghdad/Iraq

Published

2021-05-17

How to Cite

Abbas Al-Zubaidi, S. F. ., & Hussain, S. O. . (2021). Study the Reproductive Pattern of Different Breeds Bitches in Iraq by estimation of Kisspeptin and detection ovulation time. Indian Journal of Forensic Medicine & Toxicology, 15(3), 4345-4353. https://doi.org/10.37506/ijfmt.v15i3.15974