Genotyping of Cryptosporidium Parvum by PCR-Sequencing Analysis for The 70 Heat Shock Protein (HSP70) Gene and Dihydrofolate Reductase (DHFR) Gene
DOI:
https://doi.org/10.37506/ijfmt.v15i3.16246Keywords:
PCR, sequencing, polymorphism, Cryptosporidium parvum, HSP70 gene, DHFR gene.Abstract
Cryptosporidium is an apicomplexan parasite that is increasingly recognized among immunocompetent hosts
as a leading cause of childhood diarrhea in lower source settings and of waterborne diarrheal outbreaks in
high income countries. C. parvum, which infects bovines, wild animals and humans, account for the majority
of human infections. Cryptosporidium oocysts are transmitted via the fecal oral route, including by person to
person spread, from contaminated food or water, or from contact with infected animals. 300 Stool samples
were collected from patients in Hilla city from different hospitals. DNA extracted from stool samples, PCR
amplification of DNA. Genotyping of HSP70 gene and DHFR Gene was performed using a polymerase
chain reaction technique, followed by DNA sequencing. Accordingly, these DNA polymorphisms were
confirmed using DNA sequencing. The sequencing results showed the presence of many SNPs determined
in the HSP70 gene and DHFR gene.
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