Isolation and Identification of Streptococcus pyogenes from Patients and Objects in Hospital Environment in Thi-Qarcity in Iraq
Keywords:GAS, 16S rRNA, Pharyngitis, Streptococcus pyogenes
This study aimed to screen Streptococcus pyogenes in the hospital and investigate the incidence of this
bacteria. The genotypes of the S.pyogenes in the study samples will be determined using 16Sr RNA and some
virulence factors(emm, scpA, speA genes).The majority of the samples (74.1%) were isolated from patients
at the emergency room and (25.9%) were from hospital environment (objects).The current study showed that
the total of 215 patients with pharyngitis were included 118 (55%) males higher than 97 (45%) females. The
patients’ ages ranged from 2 to 62 years, which were divided into four categories. The majority of patients
65 (30.1%) of patients from 3 to 6 years old, 54 (25%) of patients less than 3years, 51 (23.6%) from 7 to 11
years old, and 46 (21.3%) more than 12years. Six isolates sent for sequencing were after that submission in
NCBI-GenBank database. The results of nucleotide sequence alignment of the isolates revealed that there
are multiple point mutations, four of them appeared as a single nucleotide polymorphism (SNP) and two
appeared in duplicate. Polymorphic sites (Mutations) of 6 S.pyogenes isolates.
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