Molecular detection of vicR, vicK and 16S rRNA genes of Streptococcus mutans Isolated from Dental Caries of Iraqi’s Patient

Abstract

Background : This study was carried out to detect the distribution of Streptococcus mutans with dental
caries in Iraqi’s patient samples. Out of 100 patient’s samples only (22) with age (3-17) years old were
found to be effected with Streptococcus mutans. DNA was extracted amplified by Gradient PCR and then
sequenced. vicR was showed highly conserved with no mutation while vicK showed that these were silent
mutation and four isolates have missense mutation from eight isolate.
Material & Method : In this study, 22 from clinical S. mutans strains were isolated from caries- children
to sequence the vicR, vicK and 16s rRNA genes. Genomic DNA was extracted from S. mutans strains and
amplified using Gradient PCR. The PCR products were purified and then sequenced.
Result : The molecular study include vicR, vicK and 16S rRNA, genes by Gradient PCR. The result of
sequencing appear that no mutation in vicR genes, although in 16S rRNA present transion and tranvesion
mutation . Finally the sequencing of vicK gene appear silent mutation and four isolates have missense
mutation from eight
Conclusion the result of molecular study of vicR gene were appear no mutation in all isolates while 16S
rRNA although have transition and transvertion mutation, the Sequence of vicK gene that note more of
mutation was silent mutation but some of them was missense mutation.