Biochemical and Histopathological effects of Acetaminophen and Protective Effects of Naringin on Liver Rats

Abstract

This study was conducted to find out liver protective activity of naringin (NRG) 40-80 mg/kg body weight
(b.w.) against acetaminophen (ACN) 2g/kg induced liver damage in rats. Thirty two male rats were divided
into four groups : group1: negative control, (1 ml/kg Saline orally) group II: positive control ACN (2g/
kg), orally as single dose at first day, group III: ACN (2g/kg), orally as single dose at first day , plus NRG
(40 mg//kg) orally for (8) consecutive days, group IV: ACN (2g/kg), orally as single dose at first day , plus
NRG (80 mg//kg) orally for (8) consecutive days. All the rats were anesthetized to collect blood then killed
on the (9) day of the experiment to take the liver samples. ACN induced liver damage was proved by a
significant (P<0.01) reduction in the body weight , total protein (TP) , albumin (AB) , superoxide dismutase
(SOD) and catalase (CAT) enzymes and a significant (P<0.01) increased in liver weight, serum aspartate
transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), total bilirubin (TB), direct
bilirubin (DB) , malondialdehyde enzyme (MAD) and histopathological changes. Protective liver toxicity
effect and oxidative damage caused by ACN significantly (P<0.01) increasing in body weight, TP, AB ,SOD
and CAT and significantly (P<0.01) decreasing in liver weight , AST, ALT, ALP, TB, DB and MAD and
improving tissue morphology by a meliorative in NRG 40 , 80 mg/kg b.w. These results confirm that NRG
antioxidant effects can protect ACN induced hepatic toxicity in rats.