Molecular Detection of Helicobacter Pylori in Sheep at ALMuthana Province of Iraq

Abstract

Helicobacter pylori (H. Pylori) was discovered in 1982. Over 50% of the world population is infected by
this bacterium. H. Pylori is the main cause of gastritis, peptic ulcers, and gastric cancer in human. Foods
with animal origins play a substantial role in the transmission of H. Pylori. The present investigation was
carried out to study the molecular detection of H.pylori in stomach gastric tissue of the sheep.Collecting
of the samples occur randomly from various area of Al-Muthana province slaughter house(AL-Muthana,
AL-Khudur, AL-Rumethah)from October 2019 to February 2020 . One hundred and fifty (n 75 male, n 75
female) abomasums samples were collected and dissection in aseptic manner then stored in deep freeze
(Liquid nitrogen -196) until processing and analyzed for the presence of 16s RNA and VACA genotypes.
This study showed that the Prevalence of H. Pylori in the gastric samples of sheep at slaughterhouses
(Al-Muthana, Al-Khdur, Al-Rumethah) were (9 positives) 18 % and (7) 14%, (15) 30% respectively. The
most commonly detected genotypes in the gastric samples of slaughterhouses were 16s RNA (20.67%)
in ewes(25.33%) . AL-Rumetha was the most commonly detected H. Pylori (30%). Results showed that
gastric tissue of sheep can act as a reservoir to H. Pylori and disseminate the pathogen in feces and milk then
transmitted to human during uses of unpasteurized milk ,or meat .samples could be the potential sources of
virulent strains of H. Pylori. Application of sanitary measures in the storage, transportation and sale of meat
is essential for reducing the levels of H. Pylori cross contamination.