Gene Expression and Antioxidant Effects of Cinnamon (Cinnamomum Zeylanicum, Breyne) in Alloxan -Induced Diabetic Rats

Authors

  • Hayder Ghazi Abdulshaheed
  • Haidar K.A.Alsaedi

DOI:

https://doi.org/10.37506/ijfmt.v15i4.16891

Keywords:

Cinnamon, Antioxidant capacity,Diabetes mellitus,INS I gene.

Abstract

Background and Aim: In this study rats, a systematic analysis was conducted to examine the antioxidant
properties of cinnamon (Cinnamomum Zeylanicum, Breyne) in controlling high blood sugar levels, and a
pharmacological comparison made to investigate whether or not it had a positive effect on the antioxidant
system.
Materials and Methods: Alloxan 60 mg injected into the marginal ear veins of eighteen diabetic rats After
this diabetic animal study, the rats were randomly assigned to 4 groups. There were six animals in each of
the 4 groups: Group 1(C): Positive control, Received orally 5 ml normal saline (0.9% NaCl) daily. Group
2(D): Negative control, Diabetes rats only, received single-dose alloxan 60 mg/kg body weight. Group
3(K): Diabetes rats, received cinnamon orally 35 mg/kg body weight dissolved in 2 ml normal. Group 4(I):
Diabetes rats, was given 2 I.U/ animal of insulin subcutaneously daily.
Results: All animal groups were treated for four weeks.Blood samples were taken from these groups weekly
for biochemical analysis to estimate: Blood glucose malondialdehyde(MDA), Glutathione (GSH). The
results showed high glucose and MDA concentration associated with an increased oxidant stress alloxan
induces on diabetic animals. The statistical analysis showed that a cinnamon significant (P<0.05) reduction
in glucose, MDA,GSH comparison with positive control. The level of MDA, GSH was also significantly
reduced (P<0.05) in all period comparison with the period before treated with Cinnamon extract .there was
4.3-fold more Ins I upregulated in the Cinnamon -treated diabetic rats 35 percent of the control group were
heterozygous and three percent of the experimental group were mutant for that trait.
Conclusion: Furthermore, Cinnamon-treated diabetic rats (group K) showed a significant increase in
the activities of both enzymatic and non-enzymatic antioxidants with concomitant overexpression of
insulin I genes when compared to diabetic control rats (group D). Cinnamon contains antioxidants and
antihyperglycemic effects at the end of the experiment.

Author Biographies

Hayder Ghazi Abdulshaheed

Associate Professor, Department Animal Production, Agriculture College, Al-Qadisyah University, Iraq

Haidar K.A.Alsaedi

Assistant Professor, Department of Basic Science, Faculty of Dentistry, Al-Qadisyah University, Iraq

Published

2021-08-16

How to Cite

Hayder Ghazi Abdulshaheed, & Haidar K.A.Alsaedi. (2021). Gene Expression and Antioxidant Effects of Cinnamon (Cinnamomum Zeylanicum, Breyne) in Alloxan -Induced Diabetic Rats. Indian Journal of Forensic Medicine & Toxicology, 15(4), 1312-1320. https://doi.org/10.37506/ijfmt.v15i4.16891